A bio-hybrid DNA rotor-stator nanoengine that moves along predefined tracks.
A bio-hybrid DNA rotor-stator nanoengine that moves along predefined tracks.
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DOI：
10.1038/s41565-018-0109-z
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发表时间：
2018-06
影响因子：
38.3
通讯作者：
Famulok M
中科院分区：
文献类型：
--
作者：
Valero J;Pal N;Dhakal S;Walter NG;Famulok M
生物马达是高度复杂的蛋白质组件，由化学能驱动，产生线性或旋转运动。基于 DNA 纳米结构、生物混合设计或合成有机化学的合成发动机已经组装完毕。然而，具有消耗化学能的转子-定子单元的单向旋转仿生轮电机却难以实现。在这里，我们报告了一种生物混合纳米引擎，由催化定子组成，单向旋转互锁的 DNA 轮，由 NTP 水解提供动力。该引擎由工程 T7 RNA 聚合酶 (T7RNAP-ZIF) 组成，该酶连接到双链 (ds)DNA 纳米环，该纳米环连接到刚性旋转 dsDNA 轮。轮电机产生长的、重复的 RNA 转录本，这些转录本仍然附着在引擎上，并用于引导其沿着排列在 DNA 纳米管上的预定义单链 (ss)DNA 轨道移动。设计的简单性使得这种行走纳米引擎能够适应其他生物纳米结构，促进复杂的生物混合结构的构建，从而实现 NTP 驱动的运动。
Biological motors are highly complex protein assemblies that generate linear or rotary motion, powered by chemical energy. Synthetic motors based on DNA nanostructures, bio-hybrid designs, or synthetic organic chemistry have been assembled. However, unidirectionally rotating biomimetic wheel motors with rotor-stator units that consume chemical energy are elusive. Here we report a bio-hybrid nanoengine consisting of a catalytic stator that unidirectionally rotates an interlocked DNA wheel, powered by NTP hydrolysis. The engine consists of an engineered T7 RNA polymerase (T7RNAP-ZIF) attached to a double-stranded (ds)DNA nanoring that is catenated to a rigid rotating dsDNA wheel. The wheel motor produces long, repetitive RNA transcripts that remain attached to the engine and are used to guide its movement along predefined single-stranded (ss)DNA tracks arranged on a DNA nanotube. The simplicity of the design renders this walking nanoengine adaptable to other biological nanoarchitectures, facilitating the construction of complex bio-hybrid structures that achieve NTP-driven locomotion.
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影响因子：
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