Feeding a high-grain diet reduces the percentage of LPS clearance and enhances immune gene expression in goat liver.
Feeding a high-grain diet reduces the percentage of LPS clearance and enhances immune gene expression in goat liver.
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饲喂高谷物饮食可降低山羊肝脏中 LPS 清除率并增强免疫基因表达
DOI：
10.1186/s12917-015-0376-y
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发表时间：
2015-03-18
影响因子：
2.6
通讯作者：
Zhuang S
中科院分区：
文献类型：
--
作者：
Chang G;Zhang K;Xu T;Jin D;Seyfert HM;Shen X;Zhuang S
背景高谷物（HG）饮食对肝脏内脂多糖（LPS）清除和先天性免疫防御反应的影响尚不清楚。因此，我们进行了本研究，其中12只母山羊被随机分配到饲喂HG饮食（60%谷物，n = 6）的治疗组或饲喂低谷物饮食（LG; 40%谷物，n = 6）的对照组，持续6周。在山羊的肠系膜、门静脉、肝静脉以及一条股动脉中安装导管，用于测定肝脏中的血流量和LPS的净清除率。于第6周采集血浆和组织标本，分析促炎细胞因子、急性时相蛋白和生化指标，以及免疫应答相关基因的表达。肝脏LPS净清除率表明，HG饲料喂养提高了肝脏LPS清除率，但降低了清除的LPS占进入肝脏的总LPS的百分比。我们的研究结果表明，饲喂HG饮食增加了促炎细胞因子和急性时相蛋白的血浆浓度，并引发了全身炎症反应。此外，HG组的丙氨酸氨基转移酶、碱性磷酸酶和总胆红素的外周血血浆浓度较LG组升高。这表明，肝细胞损伤发生在6周后的HG饲料喂养。参与免疫反应和Toll样受体（TLR）4蛋白在肝脏中的基因的表达上调HG组相比，LG组，表明增加进入的LPS增强肝脏免疫防御反应，并有助于肝脏炎症反应。LPS进入肝脏的增加增强了肝脏的免疫防御反应，从而提高了LPS的清除率。然而，肝脏LPS清除率的降低可能是由于在HG饮食喂养期间形成肝细胞损伤。
BackgroundThe effects of feeding a high-grain (HG) diet on lipopolysaccharide (LPS) clearance and innate immune defence responses in the liver remain unclear. Therefore, we conducted the present study in which twelve female goats were randomly assigned to either a treatment group fed a HG diet (60% grain, n = 6) or a control group fed a low grain diet (LG; 40% grain, n = 6) for 6 weeks. Catheters were installed in the mesenteric, portal and hepatic veins, as well as one femoral artery of the goats, for determining blood flow and net clearance rate of LPS in the liver. Plasma and tissue samples were collected in the week 6 for analyzing pro-inflammatory cytokines, acute phase protein and biochemical parameters, as well as expression of genes involved in immune response.ResultHG diet feeding increased blood flow and LPS concentration in the portal vein, hepatic vein and artery. Hepatic net LPS clearance showed that HG diet feeding elevated the rate of hepatic LPS clearance, but decreased the percentage of removed LPS accounting for the total entry of LPS into the liver. Our results demonstrated that the feeding of HG diet increased plasma concentrations of pro-inflammatory cytokines and acute phase proteins and triggered a systemic inflammatory response. In addition, peripheral blood plasma concentrations of alanine aminotransferase, alkaline phosphatase and total bilirubin were increased in the HG group compared to the LG group. This indicated that the impairment of hepatocytes occurred after 6 weeks of HG diet feeding. The expression of genes involved in immune response and Toll-like receptor (TLR)4 protein in the liver was up-regulated in the HG group compared to the LG group, indicating that increased entry of LPS enhanced hepatic immune defence responses and contributed to hepatic inflammatory responses.ConclusionThese results provide insight into the capacity of the liver to clear LPS. The increased entry of LPS into liver enhanced hepatic immune defence responses, thereby elevated the rate of LPS clearance. However, the reduction of the percentage of hepatic LPS clearance could be due to the formation of hepatocyte lesion during HG diet feeding.
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影响因子：
--
作者：
KATZ, ML;BERGMAN, EN
通讯作者：
BERGMAN, EN
DOI：
10.1631/jzus.2005.b1045
发表时间：
2005-11-01
期刊：
Journal of Zhejiang University. Science. B
影响因子：
--
作者：
Gruys, E;Toussaint, M J M;Koopmans, S J
通讯作者：
Koopmans, S J
影响因子：
3.5
作者：
HUNTINGTON, GB;REYNOLDS, CK;STROUD, BH
通讯作者：
STROUD, BH
影响因子：
4.8
作者：
GEGNER, JA;ULEVITCH, RJ;TOBIAS, PS
通讯作者：
TOBIAS, PS
影响因子：
3.5
作者：
Emmanuel, D. G. V.;Dunn, S. M.;Ametaj, B. N.
通讯作者：
Ametaj, B. N.