A hybrid herpesvirus infectious vector based on Epstein-Barr virus and herpes simplex virus type 1 for gene transfer into human cells in vitro and in vivo
A hybrid herpesvirus infectious vector based on Epstein-Barr virus and herpes simplex virus type 1 for gene transfer into human cells in vitro and in vivo
复制标题
基于 Epstein-Barr 病毒和 1 型单纯疱疹病毒的杂交疱疹病毒感染载体，用于体外和体内基因转移到人体细胞中
DOI：
10.1128/jvi.70.12.8422-8430.1996
复制
发表时间：
1996
影响因子：
5.4
通讯作者：
JEAN
中科院分区：
文献类型：
--
作者：
Suming Wang;JEAN
我们已经开发了一种基于人疱疹病毒1型和4型、单纯疱疹病毒1型（HSV-1）和EB病毒（EBV）的微型病毒载体，pH 300，其携带用于质粒附加体维持的EBV序列和用于在辅助病毒和辅助细胞系存在下扩增并以多聚体形式包装到HSV-1衣壳中的HSV-1序列。将表达β-半乳糖苷酶的细菌lacZ基因插入pH 300的多克隆位点，制备pH 300-lac。包装的pH 300-lac DNA在组织培养中感染人细胞非常有效。pH 300-lac微型病毒原液用于体外感染源自乳腺癌、肺癌和肝癌的各种人细胞类型。高达95%的细胞被感染，并在感染复数为3时暴露于病毒原液后表达β-半乳糖苷酶活性。体外培养的细胞感染后基本上没有明显的细胞毒性。为了测试体内基因递送，将人肝肿瘤细胞预先植入裸鼠皮下并原位注射pH 300-lac，显示出异位基因表达的高效率。pH 300微型病毒载体是一种简单、有效的基因转移系统，在肿瘤和遗传性疾病的基因治疗中显示出潜在的应用前景。
We have developed a miniviral vector, pH300, based on the human herpesviruses 1 and 4, herpes simplex virus type 1 (HSV-1), and Epstein-Barr virus (EBV), carrying EBV sequences for plasmid episomal maintenance and HSV-1 sequences for amplification and packaging in multimeric form into HSV-1 capsids in the presence of a helper virus and helper cell line. A reporter gene, the bacterial lacZ gene, which expressed beta-galactosidase, was inserted into the multiple cloning site of pH300 to make pH300-lac. The packaged pH300-lac DNA was very efficient in infecting human cells in tissue culture. The pH300-lac miniviral stock was used to infect in vitro various human cell types derived from breast cancer, lung cancer, and liver cancer. Up to 95% of cells were infected and expressed beta-galactosidase activity after exposure to viral stock at a multiplicity of infection of 3. There was essentially no apparent cytotoxicity after infection of cultured cells in vitro. To test in vivo gene delivery, human liver tumor cells preimplanted subcutaneously in nude mice and injected in situ with pH300-lac showed high efficiency of ectopic gene expression. The pH300 miniviral vector is a simple and effective gene transfer system which shows potential for gene therapy of cancer and inherited diseases.
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DOI：
10.1073/pnas.81.12.3806
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
YATES, J;WARREN, N;SUGDEN, B
通讯作者：
SUGDEN, B
影响因子：
14.9
作者：
DELUCA, NA;SCHAFFER, PA
通讯作者：
SCHAFFER, PA
用有缺陷的单纯疱疹病毒 1 载体感染培养的中枢神经系统神经元会导致大肠杆菌 β-半乳糖苷酶的稳定表达。
DOI：
10.1073/pnas.87.3.1149
发表时间：
1990
影响因子：
11.1
作者：
Geller,AI;Freese,A
通讯作者：
Freese,A
影响因子：
56.9
作者：
GELLER, AI;BREAKEFIELD, XO
通讯作者：
BREAKEFIELD, XO
单纯疱疹病毒 DNA 的复制：复制识别信号在有缺陷的病毒基因组内的定位。
DOI：
10.1073/pnas.78.2.742
发表时间：
1981
影响因子：
11.1
作者：
Vlazny,DA;Frenkel,N
通讯作者：
Frenkel,N