Effects of soy protein and isoflavones on insulin resistance and adiponectin in male monkeys.
Effects of soy protein and isoflavones on insulin resistance and adiponectin in male monkeys.
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大豆蛋白和异黄酮对雄性猴子胰岛素抵抗和脂联素的影响。
DOI：
10.1016/j.metabol.2008.04.001
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发表时间：
2008-07
影响因子：
9.8
通讯作者：
Adams, Michael R.
中科院分区：
文献类型：
--
作者：
Wagner, Janice D.;Zhang, Li;Shadoan, Melanie K.;Kavanagh, Kylie;Chen, Haiying;Tresnasari, Kristianti;Kaplan, Jay R.;Adams, Michael R.
异黄酮可能通过其众所周知的受体介导的雌激素活性影响胰岛素作用。然而，β-咖啡酮也结合与胰岛素作用密切相关的过氧化物酶体增殖物激活受体。大豆蛋白和它的三羟甲基黄酮以前已经被证明可以改善糖尿病绝经后妇女的血糖控制，并改善卵巢切除猴子的胰岛素敏感性。本报告的目的是将我们的饮食大豆蛋白研究扩展到雄性猴子，并确定大豆异黄酮对胰岛素抵抗的影响。这里报告了两项研究。第一项研究涉及91只雄性猴子，它们食用三种不同的食物，只是蛋白质来源不同（酪蛋白-乳白蛋白、低浓度乳清酸的大豆蛋白或高浓度乳清酸的大豆蛋白）。静脉葡萄糖耐量试验（IVGTT）和血浆脂联素和脂蛋白浓度测定后25个月的研究。在31个月时获得内脏脂肪样品用于评估脂联素和过氧化物酶体增殖物激活受体γ表达。第二项研究涉及8只拉丁方设计的猴子，比较了酪蛋白/乳清蛋白、高胆固醇浓度的大豆蛋白或经酒精洗涤以消耗胆固醇的大豆蛋白的饮食效果。治疗8周后，评估胰岛素敏感性和血浆脂蛋白。10周时取骨骼肌进行胰岛素受体、过氧化物酶体增殖物激活受体α（PPARα）和过氧化物酶体增殖物激活受体γ（PPARγ）含量的测定。主要的发现是，摄入含胆甾酮的大豆蛋白剂量依赖性地增加了胰岛素对葡萄糖刺激的反应，并降低了血浆脂联素，而胆甾酮耗尽的大豆蛋白降低了体重，对血浆脂联素浓度没有影响。相对于酪蛋白或含有大豆酮的大豆蛋白，去除大豆酮的肌肉PPARα和γ表达也增加。需要进一步的研究来确定高大豆蛋白饮食的这些影响所涉及的机制，并优化饮食中的大豆蛋白含量，以使男性获得最大的健康益处。
Isoflavones may influence insulin action by means of their well-known receptor-mediated estrogenic activity. However, isoflavones also bind to PPAR’s which are strongly associated with insulin action. Soy protein with its isoflavones has previously been shown to improve glycemic control in diabetic postmenopausal women and to improve insulin sensitivity in ovariectomized monkeys. The purpose of the current report was to extend our studies of dietary soy protein to male monkeys and determine effects of the soy isoflavones on insulin resistance. Two studies are reported here. Study one involved 91 male monkeys consuming three diets differing only by the source of protein (casein-lactalbumin, soy protein with a low isoflavone concentration or soy protein with a high isoflavone concentration). Intravenous glucose tolerance tests (IVGTTs) were done and plasma adiponectin and lipoprotein concentrations were determined after 25 months of study. Samples of visceral fat were obtained at 31 months for assessment adiponectin and PPARγ expression. The second study involved 8 monkeys in a Latin square design that compared the effects of diets with either casein/lactalbumin, soy protein with a high isoflavone concentration, or soy protein that was alcohol-washed to deplete the isoflavones. After eight weeks of treatment, insulin sensitivity and plasma lipoproteins were assessed. At ten weeks, skeletal muscle was biopsied for determination of insulin receptor, PPARα and PPARγ content. The major findings were that consumption of isoflavone-containing soy protein dose-dependently increased insulin responses to the glucose challenge and decreased plasma adiponectin while isoflavone-depleted soy protein decreased body weight and had no effect on plasma adiponectin concentrations. Muscle PPARα and γ expression was also increased with the isoflavone-depleted soy relative to either casein or soy protein containing the isoflavones. Further studies are needed to determine the mechanisms involved in these effects of a high soy isoflavone diet and to optimize dietary isoflavone content for maximal health benefits in males.
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影响因子：
4.2
作者：
Goodman-Gruen, D;Kritz-Silverstein, D
通讯作者：
Kritz-Silverstein, D
影响因子：
3.6
作者：
Morifuji, Masashi;Sanbongi, Chiaki;Sugiura, Katsumi
通讯作者：
Sugiura, Katsumi
影响因子：
120.7
作者：
Mokdad, AH;Ford, ES;Marks, JS
通讯作者：
Marks, JS
影响因子：
8.9
作者：
Lihn, AS;Pedersen, SB;Richelsen, B
通讯作者：
Richelsen, B
影响因子：
15.9
作者：
COSTRINI, NV;KALKHOFF, RK
通讯作者：
KALKHOFF, RK