The discovery of phiAGATE, a novel phage infecting Bacillus pumilus, leads to new insights into the phylogeny of the subfamily Spounavirinae.
The discovery of phiAGATE, a novel phage infecting Bacillus pumilus, leads to new insights into the phylogeny of the subfamily Spounavirinae.
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DOI：
10.1371/journal.pone.0086632
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Goździcka-Józefiak A
中科院分区：
文献类型：
--
作者：
Barylski J;Nowicki G;Goździcka-Józefiak A
噬菌体芽孢杆菌是从波兰西部富营养化的戈雷基湖水域中分离到的一种新的肌病毒。噬菌体感染短小芽孢杆菌，短小芽孢杆菌是在上述水库中常见的一种细菌。对噬菌体基因组(149844个碱基对)的分析得到了204个预测的蛋白质编码序列(CDS)，其中53个可以进行功能注释。进一步的研究表明，该噬菌体是Spounavirinae亚家族中一个以前未被描述的噬菌体群(在本研究中，我们将其称为“巴士底狱群”)的成员。在这里，我们证明了这些病毒构成了SpounaVirinae系统发育树的一个独特的分支，与Twortlike病毒和SpounalikeVirus属的噬菌体相似性有限。事实证明，将巴士底狱群中的噬菌体归入任何目前被接受的属都是极其困难的，这引发了人们对该亚科目前分类安排的有效性的担忧。
The Bacillus phage phiAGATE is a novel myovirus isolated from the waters of Lake Góreckie (a eutrophic lake in western Poland). The bacteriophage infects Bacillus pumilus, a bacterium commonly observed in the mentioned reservoir. Analysis of the phiAGATE genome (149844 base pairs) resulted in 204 predicted protein-coding sequences (CDSs), of which 53 could be functionally annotated. Further investigation revealed that the bacteriophage is a member of a previously undescribed cluster of phages (for the purposes of this study we refer to it as “Bastille group”) within the Spounavirinae subfamily. Here we demonstrate that these viruses constitute a distinct branch of the Spounavirinae phylogenetic tree, with limited similarity to phages from the Twortlikevirus and Spounalikevirus genera. The classification of phages from the Bastille group into any currently accepted genus proved extremely difficult, prompting concerns about the validity of the present taxonomic arrangement of the subfamily.
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