Typing of canine parvovirus strains circulating in Brazil between 2008 and 2010.
Typing of canine parvovirus strains circulating in Brazil between 2008 and 2010.
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DOI：
10.1016/j.virusres.2012.01.001
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发表时间：
2012-04
期刊：
影响因子：
5
通讯作者：
Canal CW
中科院分区：
文献类型：
--
作者：
Pinto LD;Streck AF;Gonçalves KR;Souza CK;Corbellini ÂO;Corbellini LG;Canal CW
在巴西流行的犬细小病毒株被检测和鉴定。粪便样本经PCR分析并测序。►对流传的变异进行了系统发育分析。CPV-2c是2008年至2010年在巴西流行的主要亚型。目前流行的犬细小病毒（CPV-2）毒株的检测和特征对于了解病毒进化和制定控制其传播的措施至关重要。采用聚合酶链反应（PCR）对144只狗的粪便进行CPV-2检测，结果显示29.2%（42/144）的狗粪便CPV-2阳性。在42株阳性菌株中，71.4%（30只）的狗有出血性胃肠炎的症状。阳性菌株VP2基因583 bp片段测序结果显示，78.6%（33/42）为2c型，19%（8/42）为2b型，2.4%（1/42）为2a型。对巴西犬群中流行的变异进行的系统发育分析表明，它们与在其他国家发现的变异非常相似，2c型已成为巴西流行的主要型。
► Canine parvovirus strains circulating in Brazil were detected and characterised. ► Stool samples were analysed by PCR and sequenced. ► Phylogenetic analysis of the variants circulating was performed. ► CPV-2c was the predominant subtype circulating in Brazil between years 2008 and 2010. Detection and characterisation of the canine parvovirus (CPV-2) strains that are currently circulating are essential for the understanding of viral evolution and the development of measures to control its spread. In the present study, stool samples from 144 dogs were analysed by polymerase chain reaction (PCR) for CPV-2, and 29.2% (42/144) of them were positive. From the 42 positive strains, 71.4% (30) of the dogs had signs of haemorrhagic gastroenteritis. The sequencing of the 583 bp fragment of the VP2 gene from the positive strains identified 78.6% (33/42) of them as type 2c, 19% (8/42) as type 2b and 2.4% (1/42) as type 2a. A phylogenetic analysis of the variants circulating in the canine population of Brazil showed that they are very similar to those found in other countries and type 2c has become the predominant type circulating in Brazil.
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影响因子：
3.3
作者：
Perez, Ruben;Francia, Lourdes;Hernandez, Martfn
通讯作者：
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影响因子：
1.5
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DOI：
10.1590/s0102-09351999000600004
发表时间：
1999-12-01
影响因子：
0.4
作者：
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通讯作者：
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影响因子：
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作者：
Nakamura, M;Tohya, Y;Akashi, H
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Akashi, H