Single stream inertial focusing in a straight microchannel.
Single stream inertial focusing in a straight microchannel.
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DOI：
10.1039/c4lc01462f
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发表时间：
2015-04-21
期刊：
影响因子：
6.1
通讯作者：
Papautsky I
中科院分区：
文献类型：
--
作者：
Wang X;Zandi M;Ho CC;Kaval N;Papautsky I
在过去的二十年中，微流体技术在精确排列流体中的细胞或微粒方面具有重要价值。微流体技术使用外力或鞘流来聚焦颗粒样品，并且面临复杂的仪器设计和有限的通量的挑战。新兴的惯性微流体领域带来了单位置聚焦功能，其吞吐量比以前高出几个数量级。然而，大多数惯性微流体聚焦器依赖于横截面流动诱导的拖曳力来实现单位置聚焦，这不可避免地使装置设计和操作复杂化。在这项工作中，我们提出了一种惯性微流体聚焦器，使用惯性升力作为唯一的驱动力，将微粒聚焦到一个单一的位置。我们展示了不同尺寸的微珠和细胞的单位置聚焦，效率为95~100%，而不需要二次流、鞘流或外力。我们进一步将该装置与激光计数系统集成，以形成无鞘流式细胞仪，并展示了2200珠/s的吞吐量和7%的变异系数的微珠计数。通过我们的集成细胞仪系统后，细胞可以完全回收并保持活力。我们的方法提供了许多优点，包括基本原理和几何形状的简单性，设计，修改和集成的方便性，不同样品聚焦的灵活性，与真实世界细胞样品的高度兼容性以及高精度和高通量的单位置聚焦。
In the past two decades, microfluidics has become of great value in precisely aligning cells or microparticles within fluids. Microfluidic techniques use either external forces or sheath flow to focus particulate samples, and face the challenges of complex instrumentation design and limited throughput. The burgeoning field of inertial microfluidics brings single-position focusing functionality at throughput orders of magnitude higher than previously available. However, most inertial microfluidic focusers rely on cross-sectional flow-induced drag force to achieve single-position focusing, which inevitably complicates the device design and operation. In this work, we present an inertial microfluidic focuser that uses inertial lift force as the only driving force to focus microparticles into a single position. We demonstrate single-position focusing of different sized microbeads and cells with 95~100% efficiency, without the need for secondary flow, sheath flow or external forces. We further integrate this device with a laser counting system to form a sheathless flow cytometer, and demonstrated counting of microbeads with 2200 beads/s throughput and 7% coefficient of variation. Cells can be completely recovered and remain viable after passing our integrated cytometry system. Our approach offers a number of benefits, including simplicity in fundamental principle and geometry, convenience in design, modification and integration, flexibility in focusing of different samples, high compatibility with real-world cellular samples as well as high-precision and high-throughput single-position focusing.
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影响因子：
4.1
作者：
Lee, Myung Gwon;Choi, Sungyoung;Park, Je-Kyun
通讯作者：
Park, Je-Kyun
影响因子：
14.8
作者：
通讯作者：
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影响因子：
6.1
作者：
Chen Y;Nawaz AA;Zhao Y;Huang PH;McCoy JP;Levine SJ;Wang L;Huang TJ
通讯作者：
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影响因子：
6.1
作者：
Kuntaegowdanahalli, Sathyakumar S.;Bhagat, Ali Asgar S.;Papautsky, Ian
通讯作者：
Papautsky, Ian
影响因子：
64.8
作者：
MacDonald, MP;Spalding, GC;Dholakia, K
通讯作者：
Dholakia, K