High-resolution cytogenetic map for the African malaria vector Anopheles gambiae.

High-resolution cytogenetic map for the African malaria vector Anopheles gambiae.
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DOI:
10.1111/j.1365-2583.2010.01025.x
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发表时间:
2010-10
影响因子:
2.6
通讯作者:
Sharakhov IV
Sharakhov IV
中科院分区:
农林科学2区
文献类型:
--
作者:
George P;Sharakhova MV;Sharakhov IV

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细胞遗传学和物理图谱对于基因组序列的精确组装、染色体区域的功能表征以及群体遗传和分类研究是不可或缺的。我们已经创建了冈比亚按蚊的一个新的细胞遗传图谱,通过使用高压挤压技术,提高了整个波段的清晰度。为了将染色体区域与基因组序列连接起来,我们基于302个BAC标记,cDNA克隆和pcr扩增的基因片段,以大约0.5-1 Mb的间隔将基因组坐标附加到染色体带和带间。此外,我们在图谱上放置了7个常见多态性反转的断点,并描述了用于臂和反转识别的染色体标记。该图谱的改进分辨率可用于进一步加强物理制图,改进基因组组装,并促进疟疾病媒的表观基因组研究。
Cytogenetic and physical maps are indispensible for precise assembly of genome sequences, functional characterization of chromosomal regions, and population genetic and taxonomic studies. We have created a new cytogenetic map for Anopheles gambiae by using a high-pressure squash technique that increases overall band clarity. To link chromosomal regions to the genome sequence, we attached genome coordinates, based on 302 markers of BAC, cDNA clones, and PCR-amplified gene fragments, to the chromosomal bands and interbands at approximately a 0.5-1 Mb interval. In addition, we placed the breakpoints of seven common polymorphic inversions on the map and described the chromosomal landmarks for the arm and inversion identification. The map's improved resolution can be used to further enhance physical mapping, improve genome assembly, and stimulate epigenomic studies of malaria vectors.
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