Magnesium Increases Homoeologous Crossover Frequency During Meiosis in ZIP4 (Ph1 Gene) Mutant Wheat-Wild Relative Hybrids.
Magnesium Increases Homoeologous Crossover Frequency During Meiosis in ZIP4 (Ph1 Gene) Mutant Wheat-Wild Relative Hybrids.
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DOI：
10.3389/fpls.2018.00509
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发表时间：
2018
影响因子：
5.6
通讯作者：
Moore G
中科院分区：
文献类型：
--
作者：
Rey MD;Martín AC;Smedley M;Hayta S;Harwood W;Shaw P;Moore G
野生近缘种为小麦育种提供了重要的有用性状来源。小麦和野生近缘杂种已被广泛用于育种计划，以将这些性状引入小麦。然而，由于小麦与野生近缘染色体之间的同源异交（CO）频率低，限制了基因渗入的成功。在染色体5 B（ph 1b）上携带70 Mb缺失的小麦与野生近缘种之间的杂交，已被利用来增加同源异源CO的水平，允许它们的染色体之间的染色体交换。在ph 1b-黑麦杂交种中，CO数量从平均每个细胞1个CO增加到7个CO。通过用Hoagland溶液处理植物，这些ph 1b杂种中的CO数可以进一步增加到平均每个细胞12个CO。最近，研究表明，染色体5 B上的主要减数分裂交换基因ZIP 4（TaZIP 4-B2）在70 Mb缺失内负责限制小麦-野生型近缘杂种中的同源异源COs，从而确认ph 1b表型为完整的Tazip 4-B2缺失突变体（Tazip 4-B2 ph 1b）。在这项研究中，我们已经确定了负责增加Tazip 4-B2 ph 1b突变体-黑麦杂交种交叉频率的特定Hoagland溶液成分，并将分析扩展到Tazip 4-B2 TILLING和CRISPR突变体-Ae variabilis杂交种。在不存在每种Hoagland溶液常量营养素（NH 4 H2 PO 4、KNO 3、Ca（NO3）2·4 H2O或MgSO 4·7 H2O）的情况下，分析减数分裂中期I的交叉频率。当Mg ~（2+）离子不存在时，观察到同型半胱氨酸CO频率显著降低。在所有分析的杂交种中观察到homoeetrach CO频率的显着增加，当植物用1 mM Mg 2+溶液灌溉时。这些观察结果表明，镁补充剂在提高野生近缘种遗传物质渗入小麦的成功率方面发挥了作用。
Wild relatives provide an important source of useful traits in wheat breeding. Wheat and wild relative hybrids have been widely used in breeding programs to introduce such traits into wheat. However, successful introgression is limited by the low frequency of homoeologous crossover (CO) between wheat and wild relative chromosomes. Hybrids between wheat carrying a 70 Mb deletion on chromosome 5B (ph1b) and wild relatives, have been exploited to increase the level of homoeologous CO, allowing chromosome exchange between their chromosomes. In ph1b-rye hybrids, CO number increases from a mean of 1 CO to 7 COs per cell. CO number can be further increased up to a mean of 12 COs per cell in these ph1b hybrids by treating the plants with Hoagland solution. More recently, it was shown that the major meiotic crossover gene ZIP4 on chromosome 5B (TaZIP4-B2) within the 70 Mb deletion, was responsible for the restriction of homoeologous COs in wheat-wild relative hybrids, confirming the ph1b phenotype as a complete Tazip4-B2 deletion mutant (Tazip4-B2 ph1b). In this study, we have identified the particular Hoagland solution constituent responsible for the increased chiasma frequency in Tazip4-B2 ph1b mutant-rye hybrids and extended the analysis to Tazip4-B2 TILLING and CRISPR mutant-Ae variabilis hybrids. Chiasma frequency at meiotic metaphase I, in the absence of each Hoagland solution macronutrient (NH4 H2PO4, KNO3, Ca (NO3)2·4H2O or Mg SO4·7H2O) was analyzed. A significant decrease in homoeologous CO frequency was observed when the Mg2+ ion was absent. A significant increase of homoeologous CO frequency was observed in all analyzed hybrids, when plants were irrigated with a 1 mM Mg2+ solution. These observations suggest a role for magnesium supplementation in improving the success of genetic material introgression from wild relatives into wheat.
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DOI：
10.1007/s11032-017-0700-2
发表时间：
2017
期刊：
Molecular breeding : new strategies in plant improvement
影响因子：
--
作者：
Rey MD;Martín AC;Higgins J;Swarbreck D;Uauy C;Shaw P;Moore G
通讯作者：
Moore G
影响因子：
64.8
作者：
RILEY, R;CHAPMAN, V
通讯作者：
CHAPMAN, V
影响因子：
1.1
作者：
Kousaka, Ryota;Endo, Takashi R.
通讯作者：
Endo, Takashi R.
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F
影响因子：
5.1
作者：
Hellens, RP;Edwards, EA;Mullineaux, PM
通讯作者：
Mullineaux, PM