Contribution of mutation, recombination, and gene conversion to chicken MHC-B haplotype diversity.
Contribution of mutation, recombination, and gene conversion to chicken MHC-B haplotype diversity.
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突变，重组和基因转化对鸡肉MHC-B单倍型多样性的贡献。
DOI：
10.4049/jimmunol.181.5.3393
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发表时间：
2008-09-01
期刊：
影响因子：
--
通讯作者：
Shiina T
中科院分区：
文献类型：
--
作者：
Hosomichi K;Miller MM;Goto RM;Wang Y;Suzuki S;Kulski JK;Nishibori M;Inoko H;Hanzawa K;Shiina T
Mhc是一个高度保守的基因区域，由于其基因家族的快速进化而引起遗传学家的兴趣，高水平的Mhc遗传多样性通常存在于种群中。鸡Mhc是相当大的兴趣，因为强大的，可重复的传染病协会发现特定的Mhc-B单倍型的焦点。Mhc-B单倍型的序列数据一直缺乏，从而阻碍了系统地解决Mhc-B区域内哪些基因有助于明确的Mhc-B相关疾病反应的努力。为了更好地了解产生和维持Mhc-B区域基因组多样性的遗传因素，我们确定了59 kb区域内14个Mhc-B单倍型的完整基因组序列，该区域包括从BG 1到BF 2的14个基因位点。我们比较了序列比对，遗传学和基因组分析方法。我们确定了基因结构变化，同义和非同义多态性，插入和缺失，等位基因重排或交换，有助于单倍型多样性。MHC-B单倍型多样性似乎是由许多突变事件产生的。我们发现证据表明，除了核苷酸突变之外，一些Mhc-B单倍型还通过完整和部分等位基因转换和同源相互重组衍生。这些数据提供了一个框架，进一步分析这14个单倍型和额外的单倍型分离和野生和驯化的鸡种群中的发展中发现的疾病协会。
The Mhc is a highly conserved gene region especially interesting to geneticists because of the rapid evolution of gene families found within it. High levels of Mhc genetic diversity often exist within populations. The chicken Mhc is the focus of considerable interest because of the strong, reproducible infectious disease associations found with particular Mhc-B haplotypes. Sequence data for Mhc-B haplotypes have been lacking thereby hampering efforts to systematically resolve which genes within the Mhc-B region contribute to well-defined Mhc-B-associated disease responses. To better understand the genetic factors that generate and maintain genomic diversity in the Mhc-B region, we determined the complete genomic sequence for 14 Mhc-B haplotypes across a region of 59 kb that encompasses 14 gene loci ranging from BG1 to BF2. We compared the sequences using alignment, phylo-genetic, and genome profiling methods. We identified gene structural changes, synonymous and non-synonymous polymorphisms, insertions and deletions, and allelic gene rearrangements or exchanges that contribute to haplotype diversity. Mhc-B haplotype diversity appears to be generated by a number of mutational events. We found evidence that some Mhc-B haplotypes are derived by whole- and partial-allelic gene conversion and homologous reciprocal recombination, in addition to nucleotide mutations. These data provide a framework for further analyses of disease associations found among these 14 haplotypes and additional haplotypes segregating and evolving in wild and domesticated populations of chickens.
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影响因子：
3.2
作者：
LAMONT, SJ;BOLIN, C;CHEVILLE, N
通讯作者：
CHEVILLE, N
影响因子：
3.2
作者：
SIMONSEN, M;CRONE, M;HALA, K
通讯作者：
HALA, K
影响因子：
56.9
作者：
BRILES, WE;BRILES, RW;STONE, HA
通讯作者：
STONE, HA
DOI：
10.1073/pnas.93.9.3958
发表时间：
1996-04-30
影响因子：
11.1
作者：
Miller, MM;Goto, RM;Bloom, SE
通讯作者：
Bloom, SE
DOI：
10.1159/000134445
发表时间：
1996-01-01
期刊：
CYTOGENETICS AND CELL GENETICS
影响因子：
--
作者：
Fillon, V;Zoorob, R;Vignal, A
通讯作者：
Vignal, A