Diurnal rhythm of human semen quality: analysis of large-scale human sperm bank data and timing-controlled laboratory study.
Diurnal rhythm of human semen quality: analysis of large-scale human sperm bank data and timing-controlled laboratory study.
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人类精液质量的昼夜节律：大规模人类精子库数据分析和时间控制实验室研究。
DOI：
10.1093/humrep/deac135
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发表时间：
2022-07-30
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我们能否确定人类精液参数的昼夜波动以及精液质量的峰值时间呢？
人类精液参数呈现出显著的昼夜波动，大多数参数在11点至15点间达到峰值。
生物钟似乎调控着各个器官的不同生理功能，但人类精液参数是否存在昼夜节律仍存在争议。
本研究利用某省级人类精子库（HSB）的病历资料，这些资料涵盖了2010年3月1日至2015年7月8日期间8点至17点采集的33430份精液样本，以分析不同时间点精液参数的变化情况。同时开展了一项实验室研究，于2019年6月6日至7月8日期间，从6名志愿者处按相同时间间隔（2天零4小时）的6个时间点采集精液样本（n = 36），在严格控制禁欲期的情况下，探究体内精液参数的昼夜波动。因此，可将大样本量的精子库研究与在24小时时间窗口内严格控制禁欲期的体内研究进行对比，以描述人类精液参数的昼夜节律。
样本取自潜在的精子库供体以及通过在社区发放传单招募的实验室研究志愿者。使用计算机辅助精子分析评估精子总数、精子浓度、精液量、前向运动能力和总运动能力。此外，在实验室研究中，通过精子染色质结构分析评估精子染色质完整性参数（DNA碎片化指数和高DNA可染性），并采用流式细胞术检测精子活力。
精子库的33430份样本显示，8点至17点间，精子总数、精子浓度、精液量、前向运动能力和总运动能力均存在时间上的变化（所有P < 0.001）。因此，根据精子库标准，用于辅助生殖技术（ART）的精液样本的合格性随时间点波动。相较于11点，每提前/推迟1小时，样本不合格风险为1.14倍。同样，在24小时时间窗口内采集的36份样本也呈现出昼夜波动。在严格控制采精前禁欲期的情况下，大多数精液参数在11点至15点间达到最理想水平。
分析中未对一些可能影响精液质量或昼夜节律的混杂因素（如能量摄入）进行调整。此外，由于本研究是在特定人群、时间和地点开展的，而波动时间可能会随条件变化而不同，因此对研究结果应谨慎看待。
这些研究结果有助于我们更精确地评估精液质量，获取更高质量的精子用于辅助生殖技术和自然受孕。
本研究得到了国家自然科学基金（81871208）和国家重点研发计划（2017YFC1002001）的支持。无利益冲突声明。
无适用内容。
Can we identify diurnal oscillations in human semen parameters as well as peak times of semen quality? Human semen parameters show substantial diurnal oscillation, with most parameters reaching a peak between 1100 and 1500 h. A circadian clock appears to regulate different physiological functions in various organs, but it remains controversial whether diurnal rhythms occur in human semen parameters. The medical record of a provincial human sperm bank (HSB) with 33 430 semen samples collected between 0800 and 1700 h from 1 March 2010 to 8 July 2015 was used to analyze variation in semen parameters among time points. A laboratory study was conducted to collect semen samples (n = 36) from six volunteers at six time points with identical time intervals (2 days plus 4 h) between 6 June and 8 July in 2019, in order to investigate the diurnal oscillation of semen parameters in vivo, with a strictly controlled abstinence period. Therefore, the sperm bank study with a large sample size and the in vivo study with a strictly controlled abstinence period in a 24-h time window could be compared to describe the diurnal rhythms in human semen parameters. Samples were obtained from potential HSB donors and from participants in the laboratory study who were volunteers, recruited by flyers distributed in the community. Total sperm count, sperm concentration, semen volume, progressive motility and total motility were assessed using computer-aided sperm analysis. In addition, sperm chromatin integrity parameters (DNA fragmentation index and high DNA stainability) were assessed by the sperm chromatin structure assay, and sperm viability was measured with flow cytometry in the laboratory study. The 33 430 samples from the HSB showed a temporal variation in total sperm count, sperm concentration, semen volume, progressive motility and total motility (all P < 0.001) between 0800 and 1700 h. Consequently, the eligibility of semen samples for use in ART, based on bank standards, fluctuated with time point. Each hour earlier/later than 1100 h was associated with 1.14-fold risk of ineligibility. Similarly, the 36 samples taken during the 24-h time window showed diurnal oscillation. With the pre-collection abstinence period strictly controlled, most semen parameters reached the most favorable level between 1100 and 1500 h. Some of the possible confounding factors, such as energy intake, which might influence semen quality or diurnal rhythms, were not adjusted for in the analyses. In addition, the findings should be considered with caution because the study was conducted in a specific population, time and place, while the timing of oscillations could differ with changing conditions. The findings could help us to estimate semen quality more precisely and to obtain higher quality sperm for use in ART and in natural conception. This study was supported by the National Natural Science Foundation of China (81871208) and National Key R&D Program of China (2017YFC1002001). There are no conflicts of interest to declare. N/A.
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影响因子：
6.1
作者：
Li, Yafei;Lin, Hui;Cao, Jia
通讯作者：
Cao, Jia
影响因子：
3.2
作者：
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Lopes, Flavio Guiselli
影响因子：
6.1
作者：
Castilla, JA;Alvarez, C;Martínez, L
通讯作者：
Martínez, L
昼夜节律的代谢和能量学的融合。
DOI：
10.1126/science.1195027
发表时间：
2010-12-03
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bass J;Takahashi JS
通讯作者：
Takahashi JS
影响因子：
6.1
作者：
Calhaz-Jorge, C.;de Geyter, C.;Baranowski, Richard
通讯作者：
Baranowski, Richard