Transcriptomic analysis of fiber strength in upland cotton chromosome introgression lines carrying different Gossypium barbadense chromosomal segments.
Transcriptomic analysis of fiber strength in upland cotton chromosome introgression lines carrying different Gossypium barbadense chromosomal segments.
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携带不同海岛棉染色体片段的陆地棉染色体渗入系纤维强度的转录组分析
DOI：
10.1371/journal.pone.0094642
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zhang T
中科院分区：
文献类型：
--
作者：
Fang L;Tian R;Chen J;Wang S;Li X;Wang P;Zhang T
纤维强力是决定棉花纤维品质的关键性状，与次生细胞壁的合成密切相关。为了了解纤维强度的机制，我们比较了不同的海岛棉染色体导入系(CSIL)的纤维转录本，这些CSIL的纤维强度高于它们的受体陆地棉。TM-1。在CSIL-35431和CSIL-31010之间共检测到18,288个差异表达基因(DIG)，这两个CSIL是纤维较强的CSIL和次生细胞壁合成过程中的TM-1。功能分类和富集分析表明，这些DEG对次生细胞壁生物发生、葡醛酸木聚糖生物合成、纤维素生物合成、糖介导的信号转导途径和脂肪酸生物合成具有丰富的作用。途径分析表明，这些DEG参与淀粉和蔗糖代谢(328个基因)、糖酵解/糖异生(122个基因)、苯丙烷生物合成(101个基因)和氧化磷酸化(87个基因)等。此外，MYB和NAC型转录因子基因在CSIL和TM-1中的表达也有显著差异。与CSIL-31134、CSIL-35431和CSIL-31010不同的是，CSIL-35368中有许多与脂肪酸降解和生物合成相关的基因，也有与碳水化合物代谢相关的基因下调。CSIL的代谢途径分析表明，不同的途径发生了变化，其中一些途径在同一发育阶段发生了一些变化。我们的结果加深了我们对碳水化合物代谢途径和次生细胞壁生物合成影响纤维强度的理解，并提示更多的基因和/或途径与复杂的纤维强度形成过程有关。
Fiber strength is the key trait that determines fiber quality in cotton, and it is closely related to secondary cell wall synthesis. To understand the mechanism underlying fiber strength, we compared fiber transcriptomes from different G. barbadense chromosome introgression lines (CSILs) that had higher fiber strengths than their recipient, G. hirsutum acc. TM-1. A total of 18,288 differentially expressed genes (DEGs) were detected between CSIL-35431 and CSIL-31010, two CSILs with stronger fiber and TM-1 during secondary cell wall synthesis. Functional classification and enrichment analysis revealed that these DEGs were enriched for secondary cell wall biogenesis, glucuronoxylan biosynthesis, cellulose biosynthesis, sugar-mediated signaling pathways, and fatty acid biosynthesis. Pathway analysis showed that these DEGs participated in starch and sucrose metabolism (328 genes), glycolysis/gluconeogenesis (122 genes), phenylpropanoid biosynthesis (101 genes), and oxidative phosphorylation (87 genes), etc. Moreover, the expression of MYB- and NAC-type transcription factor genes were also dramatically different between the CSILs and TM-1. Being different to those of CSIL-31134, CSIL-35431 and CSIL-31010, there were many genes for fatty acid degradation and biosynthesis, and also for carbohydrate metabolism that were down-regulated in CSIL-35368. Metabolic pathway analysis in the CSILs showed that different pathways were changed, and some changes at the same developmental stage in some pathways. Our results extended our understanding that carbonhydrate metabolic pathway and secondary cell wall biosynthesis can affect the fiber strength and suggested more genes and/or pathways be related to complex fiber strength formation process.
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影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
5.4
作者：
Hinchliffe, Doug J.;Meredith, William R.;Triplett, Barbara A.
通讯作者：
Triplett, Barbara A.
影响因子：
11.6
作者：
Brown, DM;Zeef, LAH;Turner, SR
通讯作者：
Turner, SR
影响因子：
7.4
作者：
MEINERT, MC;DELMER, DP
通讯作者：
DELMER, DP
影响因子：
--
作者：
Bolton JJ;Soliman KM;Wilkins TA;Jenkins JN
通讯作者：
Jenkins JN