Nerve growth factor continuously elevates in a rat rotator cuff tear model.
Nerve growth factor continuously elevates in a rat rotator cuff tear model.
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在大鼠肩袖撕裂模型中，神经生长因子持续升高。
DOI：
10.1016/j.jse.2018.06.030
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Takaso M
中科院分区：
文献类型：
--
作者：
Nagura N;Kenmoku T;Uchida K;Nakawaki M;Inoue G;Takaso M
背景神经生长因子（NGF）在骨关节炎疼痛和腰痛中起着关键作用。肩袖撕裂 (RCT) 通常与严重的肩部疼痛有关。然而，NGF在RCT中的作用仍有待充分了解。方法将大鼠分为假手术组和RCT组。在不同天从假手术组和 RCT 组收获肩袖，用于 Tnfa、Ngf、Il1b 和 Cox2 表达的逆转录聚合酶链反应分析。还在第 1 天和第 14 天收获假手术组和 RCT 组的肩袖，用于酶联免疫吸附测定和免疫组织化学，以评估 NGF 蛋白水平和定位。用大鼠重组肿瘤坏死因子 (TNF)-α 刺激肩袖来源的细胞，以研究 TNF-α 参与 NGF 表达的调节。结果 RCT 组中 Tnfa 和 Ngf 信使 RNA 水平在 1 天内升高。值得注意的是，RCT手术后Tnfa和Ngf上调持续长达56天，而Il1和Cox2表达显着降低。 RCT组NGF水平在第1天和第14天显着高于假手术组。第1天和第14天，层状撕裂表面衬里的某些炎症细胞和滑膜样细胞分别呈NGF阳性。TNF-α刺激后，肩袖来源的细胞中Ngf信使RNA水平显着升高。结论RCT中NGF水平持续升高，主要受TNF-α调节。因此，NGF 可能代表调节 RCT 疼痛疗法的潜在靶点。
BackgroundNerve growth factor (NGF) plays a key role in osteoarthritic pain and low back pain. Rotator cuff tear (RCT) is often associated with severe shoulder pain. However, the role of NGF in RCT remains to be fully understood.MethodsRats were divided into sham and RCT groups. The rotator cuff was harvested from the sham and RCT groups on various days for reverse transcription-polymerase chain reaction analysis ofTnfa,Ngf,Il1b, andCox2expression. Rotator cuffs from the sham and RCT groups were also harvested at 1 and 14 days for enzyme-linked immunosorbent assay and immunohistochemistry to assess NGF protein levels and localization. Rotator cuff–derived cells were stimulated with rat recombinant tumor necrosis factor (TNF)-α to investigate the involvement of TNF-α in the regulation of NGF expression.ResultsTnfaandNgfmessenger RNA levels increased within 1 day in the RCT group. Notably,TnfaandNgfupregulation persisted for up to 56 days after the RCT surgery, whileIl1bandCox2expression was significantly reduced. NGF levels in the RCT group were significantly higher than those in the sham operation group on days 1 and 14. Certain inflammatory cells and synovial-like cells lining the surface of the laminated tears were NGF-positive on days 1 and 14, respectively.Ngfmessenger RNA levels increased significantly in rotator cuff–derived cells after TNF-α stimulation.ConclusionNGF levels are continuously elevated in RCT, which is mainly regulated by TNF-α. NGF may thus represent a potential target for therapies that modulate RCT pain.
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DOI：
--
发表时间：
2001
期刊：
The Kobe journal of medical sciences
影响因子：
--
作者：
H. Sakai;K. Fujita;Y. Sakai;K. Mizuno
通讯作者：
K. Mizuno
影响因子：
--
作者：
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通讯作者：
LEVIMONTALCINI, R
影响因子：
3
作者：
Blaine, TA;Kim, YS;Bigliani, LU
通讯作者：
Bigliani, LU
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影响因子：
2.6
作者：
W. Wober;VW Rahlfs;N. Büchl;A. Grässle;A. Macciocchi
通讯作者：
A. Macciocchi
影响因子：
7.4
作者：
Katz, Nathaniel;Borenstein, David G.;Brown, Mark T.
通讯作者：
Brown, Mark T.