In vitro characterization of hierarchical 3D scaffolds produced by combining additive manufacturing and thermally induced phase separation.
In vitro characterization of hierarchical 3D scaffolds produced by combining additive manufacturing and thermally induced phase separation.
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通过结合增材制造和热致相分离制备的分级三维支架的体外特性
DOI：
10.1080/09205063.2020.1841535
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
James PF
中科院分区：
文献类型：
--
作者：
Yousefi AM;Powers J;Sampson K;Wood K;Gadola C;Zhang J;James PF
本文报道了我们制备聚乳酸-羟基乙酸（PLGA）和纳米羟基磷灰石（nHA）分层支架的混合工艺，通过扫描电镜分析了它们的内部结构，并给出了我们在体外培养MC3T3-E1细胞0和21天的增殖和碱性磷酸酶活性（ALP）的结果。这些支架采用增材制造（AM）和热诱导相分离（TIPS）技术相结合的方法制备。在TIPS过程中，以1.5°C/min的速度缓慢冷却，使整个支架的温度均匀，因此，孔径范围相对均匀。在这项研究中，我们制作了十种不同的支架组成和拓扑结构。这些支架的直径分别为~300 μm、~380 μm和~460 μm，由嵌入的多孔3d标绘聚乙二醇（PEG）基质提取而成。其他实验因素包括不同的TIPS温度（- 20°C， - 10°C和0°C），以及不同的PLGA浓度（8%，10%和12% w/v）和nHA含量（0%，10%和20% w/w）。我们的结果表明，几乎所有这些大孔/微孔支架在21天内支持细胞生长。然而，一些支架在支持细胞增殖和分化方面存在显著差异。本文介绍了我们的体外细胞培养0和21天的结果。我们的最佳支架孔隙率为~90%，模量为~5.2 MPa， nHA含量为20%，在第0天的细胞粘附率为~29%，在体外培养的21天内保持细胞增殖和ALP活性。因此，使用增材制造和设计实验来优化支架的制造参数，从而获得了比大多数其他使用TIPS的研究更优越的机械性能。
This paper reports on the hybrid process we have used for producing hierarchical scaffolds made of poly(lactic-co-glycolic) acid (PLGA) and nanohydroxyapatite (nHA), analyzes their internal structures via scanning electron microscopy, and presents the results of our in vitro proliferation of MC3T3-E1 cells and alkaline phosphatase activity (ALP) for 0 and 21 days. These scaffolds were produced by combining additive manufacturing (AM) and thermally induced phase separation (TIPS) techniques. Slow cooling at a rate of 1.5 °C/min during the TIPS process was used to enable a uniform temperature throughout the scaffolds, and therefore, a relatively uniform pore size range. We produced ten different scaffold compositions and topologies in this study. These scaffolds had macrochannels with diameters of ~300 μm, ~380 μm, and ~460 μm, generated by the extraction of embedded porous 3D-plotted polyethylene glycol (PEG) matrices. The other experimental factors included different TIPS temperatures (−20 °C, −10 °C, and 0 °C), as well as varying PLGA concentrations (8%, 10%, and 12% w/v) and nHA content (0%, 10%, and 20% w/w). Our results indicated that almost all these macro/microporous scaffolds supported cell growth over the period of 21 days. Nevertheless, significant differences were observed among some scaffolds in terms of their support of cell proliferation and differentiation. This paper presents the results of our in vitro cell culture for 0 and 21 days. Our optimal scaffold with a porosity of ~90%, a modulus of ~5.2 MPa, and a nHA content of 20% showed a cell adhesion of ~29% on day 0 and maintained cell proliferation and ALP activity over the 21-day in vitro culture. Hence, the use of additive manufacturing and designed experiments to optimize the scaffold fabrication parameters resulted in superior mechanical properties that most other studies using TIPS.
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影响因子：
14
作者：
Abagnale, Giulio;Steger, Michael;Wagner, Wolfgang
通讯作者：
Wagner, Wolfgang
DOI：
10.1007/s10856-015-5453-z
发表时间：
2015-02-01
影响因子：
3.7
作者：
Akbarzadeh, Rosa;Minton, Joshua A.;Yousefi, Azizeh-Mitra
通讯作者：
Yousefi, Azizeh-Mitra
DOI：
10.1007/978-1-60761-999-4_18
发表时间：
2011-01-01
期刊：
MESENCHYMAL STEM CELL ASSAYS AND APPLICATIONS
影响因子：
--
作者：
Grayson, Warren L.;Bhumiratana, Sarindr;Vunjak-Novakovic, Gordana
通讯作者：
Vunjak-Novakovic, Gordana
影响因子：
3.2
作者：
Liu, Junyi;Zhang, Jing;Yousefi, Azizeh-Mitra
通讯作者：
Yousefi, Azizeh-Mitra
影响因子：
14
作者：
Jones, Anthony C.;Arns, Christoph H.;Knackstedt, Mark A.
通讯作者：
Knackstedt, Mark A.