Detection of Multiple Intracellular Bacterial Pathogens in Haemaphysalis flava Ticks Collected from Hedgehogs in Central China.

Detection of Multiple Intracellular Bacterial Pathogens in Haemaphysalis flava Ticks Collected from Hedgehogs in Central China.
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华中地区刺猬黄血蜱中多种细胞内细菌病原体的检测。

DOI:
10.3390/pathogens10020115
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发表时间:
2021-01-23
期刊:
Pathogens (Basel, Switzerland)
影响因子:
--
通讯作者:
Yu XJ
Yu XJ
中科院分区:
其他
文献类型:
--
作者:
Fang LZ;Lei SC;Yan ZJ;Xiao X;Liu JW;Gong XQ;Yu H;Yu XJ

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蜱传细胞内细菌病原体,包括无形体、伯内蒂柯克斯体、埃利希体和立克次体,在世界范围内引起新出现的传染病。采用 PCR 扩增从中国中部地区的刺猬身上采集的黄血蜱中这些病原体的基因。在125个样本中,包括20批卵、24只饱食的雌性和81只已蜕皮的雄性和雌性成年蜱,DNA序列和系统发育分析表明,蜱的最低感染率分别为:A. bovis 4%(5/125),C. burnetti 3.2%(4/125),E. ewingii 9.6%(12/125),E. ewingii 9.6%(12/125)。立克次体为 5.6%,包括日本立克次体 (3.2%, 4/125) 和拉氏立克次体 (2.4%, 3/125)。这些病原体在死亡的充血雌性蜱虫中的患病率(83.3%,20/24)显着高于卵(5%,1/20)和蜕皮蜱虫(8.6%,7/81)。我们的研究表明,黄蜱在华中地区可能感染多种蜱传病原体,包括无形体、伯内蒂克氏体、埃里希体和立克次体,并且这些病原体在蜱中经卵巢和跨体传播期间的流行率降低,这表明蜱可能不是真正的宿主,而只是这些蜱传病原体的载体。
Tickborne intracellular bacterial pathogens including Anaplasma, Coxiella burnetti, Ehrlichia, and Rickettsia cause emerging infectious diseases worldwide. PCR was used to amplify the genes of these pathogens in Haemaphysalis flava ticks collected from hedgehogs in Central China. Among 125 samples including 20 egg batches, 24 engorged females, and 81 molted male and female adult ticks, the DNA sequences and phylogenetic analysis showed that the minimum infection rate of the ticks was 4% (5/125) for A. bovis, 3.2% (4/125) for C. burnetti, 9.6%, (12/125) for E. ewingii, and 5.6% for Rickettsia including R. japonica (3.2%, 4/125) and R. raoultii (2.4%, 3/125), respectively. The prevalence of these pathogens was significantly higher in dead engorged females (83.3%, 20/24) than in eggs (5%, 1/20) and molted ticks (8.6%, 7/81). Our study indicated that H. flava ticks could be infected with multiple species of tickborne pathogens including Anaplasma, C. burnetti, Ehrlichia, and Rickettsia in Central China, and the prevalence of these pathogens was reduced during transovarial and transstadial transmission in ticks, suggesting that ticks may not be real reservoirs but only vectors for these tickborne pathogens.
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