Evaluation of the extraction method for the cytotoxicity testing of latex gloves.
Evaluation of the extraction method for the cytotoxicity testing of latex gloves.
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DOI：
10.3349/ymj.2005.46.4.579
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发表时间：
2005-08-31
影响因子：
2.4
通讯作者：
Park JC
中科院分区：
文献类型：
--
作者：
Baek HS;Yoo JY;Rah DK;Han DW;Lee DH;Kwon OH;Park JC
在本研究中，使用各种浸提条件测定医用乳胶手套对培养的L-929细胞的细胞毒性。根据浸提时间和温度的不同，采用3种浸提条件：1）37℃浸提24 h; 2）37℃浸提72 h; 3）50℃浸提72 h。此外，还使用了四种不同的浸提介质，即蒸馏水（DW）、DW中的9 g/l氯化钠（生理盐水）以及含或不含血清的培养基。在上述条件下，提取样品，然后在3.13 - 50%的浓度范围内进行2倍系列稀释。当在37℃下用DW或生理盐水浸提24 h或72 h时，仅50%稀释的样品对L-929细胞显示出明显的细胞毒性。此外，当手套在50℃下用DW或生理盐水浸提72 h时，未观察到细胞毒性潜力。当手套用培养基浸提时，细胞毒性明显更大，无论培养基中是否存在血清。这些结果表明，生物材料和医疗器械的细胞毒性评价应建立最佳浸提条件。
In this study, the cytotoxicity of medical latex gloves to cultured L-929 cells was determined using various extraction conditions. According to the extraction time and temperature, three types of extraction conditions were used: 1) 24 h at 37℃; 2) 72 h at 37℃; 3) 72 h at 50℃. Also, four different extraction vehicles were used, namely, distilled water (DW), 9 g/l sodium chloride (saline) in DW, and culture media with or without serum. Under the above-mentioned conditions, the samples were extracted and then 2-fold serially diluted in the concentration range 3.13 - 50%. When extracted with either DW or saline for 24 h or 72 h at 37℃, only 50% diluted samples showed distinct cytotoxicity to L-929 cells. Moreover, no cytotoxic potentials were observed when gloves were extracted with DW or saline at 50℃ for 72 h. Cytotoxicity was markedly greater when gloves were extracted with culture medium, irrespective of the presence of serum in the medium. These results suggest that optimal extraction conditions should be established for the cytotoxicity evaluations of biomaterials and medical devices.
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影响因子：
2.4
作者：
Park, JC;Park, BJ;Kwon, OH
通讯作者：
Kwon, OH
DOI：
10.1002/jbm.820290707
发表时间：
1995-07-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
NISHI, C;NAKAJIMA, N;IKADA, Y
通讯作者：
IKADA, Y
DOI：
10.1016/0267-6605(94)90085-x
发表时间：
1994-01-01
期刊：
Clinical Materials
影响因子：
--
作者：
Tsuchiya, Toshie;Ikarashi, Yoshiaki;Nakamura, Akitada
通讯作者：
Nakamura, Akitada
DOI：
10.1111/j.1464-410x.1985.tb08992.x
发表时间：
1985-01-01
期刊：
BRITISH JOURNAL OF UROLOGY
影响因子：
--
作者：
RUUTU, M;ALFTHAN, O;ANDERSSON, LC
通讯作者：
ANDERSSON, LC
DOI：
10.1002/jbm.820180914
发表时间：
1984-01-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
GRAHAM, DT;MARK, GE;MACARTHUR, EB
通讯作者：
MACARTHUR, EB