Placental Leucine Aminopeptidase as a Potential Specific Urine Biomarker for Invasive Ovarian Cancer.
Placental Leucine Aminopeptidase as a Potential Specific Urine Biomarker for Invasive Ovarian Cancer.
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DOI：
10.3390/jcm11010222
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发表时间：
2021-12-31
影响因子：
3.9
通讯作者：
Shibata K
中科院分区：
文献类型：
--
作者：
Matsukawa T;Mizutani S;Matsumoto K;Kato Y;Yoshihara M;Kajiyama H;Shibata K
背景：目前缺乏一种用于检测卵巢癌（OvCa）的非侵入性和敏感的生物标志物。我们的目的是研究尿胎盘亮氨酸氨基肽酶（P-AMP）是否可以作为OvCa的可靠生物标志物。方法：使用β-淀粉酶测定试剂盒（Serotech Co.，有限公司、札幌，Japan）对22例良性或交界性恶性卵巢肿瘤患者和18例OvCa患者的尿液进行了检测。在该测定中，加入20 mM L-甲硫氨酸，因为P-蛋氨酸是功能性的，但其他氨肽酶在该剂量的L-甲硫氨酸下被抑制。结果：卵巢癌组尿P-gp活性明显高于良性或交界性肿瘤组。以11.00 U/L为界值时，P-β 1对浸润性癌的敏感性为77.8%，特异性为95.5%。结论：虽然这项测试的有用性应该在更大的病例和对照组中得到证实，但我们的研究是第一个强调尿P-CRP作为OvCa生物标志物的重要性的研究。
Background: A non-invasive and sensitive biomarker for the detection of ovarian cancer (OvCa) is lacking. We aim to investigate if urinary placental leucine aminopeptidase (P-LAP) can serve as a reliable biomarker for OvCa. Methods: P-LAP activity was measured using a LAP assay kit (Serotech Co., Ltd., Sapporo, Japan) in the urine of 22 patients with benign or borderline malignant ovarian tumors and 18 patients with OvCa. In this assay, L-methionine was added at 20 mM because P-LAP is functional, but other aminopeptidases are inhibited at this dose of L-methionine. Results: The mean urinary P-LAP activity was significantly higher in the OvCa group than in the benign or borderline malignant tumor group. When the cut-off value of P-LAP was determined as 11.00 U/L, its sensitivity and specificity for differentiating invasive cancer were 77.8% and 95.5%, respectively. Conclusion: Although the usefulness of this test should be confirmed in a larger cohort of cases and controls, our study is the first to highlight the importance of urinary P-LAP as a biomarker for OvCa.
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影响因子：
3.2
作者：
Kobayashi, H;Nomura, S;Mizutani, S
通讯作者：
Mizutani, S
影响因子：
1.9
作者：
Nagasaka, T;Nomura, S;Mizutani, S
通讯作者：
Mizutani, S
影响因子：
--
作者：
Larrinaga, Gorka;Lopez, Jose I.;Varona, Adolfo
通讯作者：
Varona, Adolfo
影响因子：
4.7
作者：
Shibata, K;Kajiyama, H;Kikkawa, F
通讯作者：
Kikkawa, F
DOI：
10.1007/978-1-4939-6934-0_19
发表时间：
2017
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Fennelly C;Amaravadi RK
通讯作者：
Amaravadi RK