Tanfloc/heparin polyelectrolyte multilayers improve osteogenic differentiation of adipose-derived stem cells on titania nanotube surfaces.
Tanfloc/heparin polyelectrolyte multilayers improve osteogenic differentiation of adipose-derived stem cells on titania nanotube surfaces.
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DOI：
10.1016/j.carbpol.2020.117079
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发表时间：
2021-01-01
影响因子：
11.2
通讯作者：
Popat KC
中科院分区：
文献类型：
--
作者：
Sabino RM;Mondini G;Kipper MJ;Martins AF;Popat KC
在这项研究中，提出了一种使用天然生物聚合物对钛进行表面改性的策略，以改善骨愈合，促进骨科植入物快速成功的骨整合。通过阳极氧化工艺制造二氧化钛纳米管，并使用基于Tanfloc（阳离子单宁衍生物）和糖胺聚糖（肝素和透明质酸）的聚电解质多层（PEM）进一步修饰表面。扫描电子显微镜（SEM），水接触角测量，和X射线光电子能谱被用来表征表面。将脂肪干细胞（ADSCs）接种于表面，观察细胞活力、粘附和增殖情况。通过矿化和蛋白质表达测定、免疫荧光染色和SEM来诱导成骨，并评价人ADSC在表面上的成骨分化。在二氧化钛纳米管上的Tanfloc/肝素PEM提高了ADSC的成骨分化速率以及骨矿物质沉积，因此是用于骨科植入物的有前途的方法。
In this study, a surface modification strategy using natural biopolymers on titanium is proposed to improve bone healing and promote rapid and successful osseointegration of orthopedic implants. Titania nanotubes were fabricated via an anodization process and the surfaces were further modified with polyelectrolyte multilayers (PEMs) based on Tanfloc (a cationic tannin derivative) and glycosaminoglycans (heparin and hyaluronic acid). Scanning electron microscopy (SEM), water contact angle measurements, and X-ray photoelectron spectroscopy were used to characterize the surfaces. Adipose-derived stem cells (ADSCs) were seeded on the surfaces, and the cell viability, adhesion, and proliferation were investigated. Osteogenesis was induced and osteogenic differentiation of human ADSCs on the surfaces was evaluated via mineralization and protein expression assays, immunofluorescent staining, and SEM. The Tanfloc/heparin PEMs on titania nanotubes improved the rate of osteogenic differentiation of ADSCs as well as the bone mineral deposition, and is therefore a promising approach for use in orthopedic implants.
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影响因子：
--
作者：
Florencio-Silva R;Sasso GR;Sasso-Cerri E;Simões MJ;Cerri PS
通讯作者：
Cerri PS
DOI：
10.1016/j.msec.2019.109850
发表时间：
2019-10-01
影响因子：
7.9
作者：
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DOI：
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影响因子：
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作者：
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通讯作者：
Popat, Ketul C.
影响因子：
11.2
作者：
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