Molecular characterization of a strawberry FaASR gene in relation to fruit ripening.
Molecular characterization of a strawberry FaASR gene in relation to fruit ripening.
复制标题
DOI：
10.1371/journal.pone.0024649
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Lu WJ
中科院分区：
文献类型：
--
作者：
Chen JY;Liu DJ;Jiang YM;Zhao ML;Shan W;Kuang JF;Lu WJ
阿坝诱导蛋白、胁迫诱导蛋白和成熟诱导蛋白（ASR）是阿坝信号转导的下游成分。尽管ASR在植物发育和逆境响应中的作用已引起人们的广泛关注，但阿坝在分子水平上调节果实成熟的机制还不完全清楚。在本工作中，草莓ASR基因的分离和鉴定（FaASR），并针对FaASR蛋白的多克隆抗体的制备。此外，阿坝，施加到草莓的两个不同的发展阶段，对果实成熟和FaASR的表达在转录和翻译水平上的影响进行了研究。 FaASR定位于细胞质和细胞核中，含有193个氨基酸，与其他植物ASR具有共同的特征。它还在酵母中作为转录激活因子，在N-末端具有反式激活活性。在草莓果实发育过程中，内源阿坝含量、FaASR mRNA和蛋白质水平在白色果实发育期成熟开始时显著增加。更重要的是，外源阿坝处理能显著提高大绿色（LG）和W果实内源阿坝含量，促进果实成熟，同时显著提高FaASR转录本的表达和FaASR蛋白的积累。这些结果表明，FaASR可能参与草莓果实成熟。观察到的内源阿坝含量的增加，增强FaASR表达在转录和翻译水平响应阿坝处理可能部分有助于草莓果实成熟的加速。
ABA-, stress- and ripening-induced (ASR) proteins have been reported to act as a downstream component involved in ABA signal transduction. Although much attention has been paid to the roles of ASR in plant development and stress responses, the mechanisms by which ABA regulate fruit ripening at the molecular level are not fully understood. In the present work, a strawberry ASR gene was isolated and characterized (FaASR), and a polyclonal antibody against FaASR protein was prepared. Furthermore, the effects of ABA, applied to two different developmental stages of strawberry, on fruit ripening and the expression of FaASR at transcriptional and translational levels were investigated. FaASR, localized in the cytoplasm and nucleus, contained 193 amino acids and shared common features with other plant ASRs. It also functioned as a transcriptional activator in yeast with trans-activation activity in the N-terminus. During strawberry fruit development, endogenous ABA content, levels of FaASR mRNA and protein increased significantly at the initiation of ripening at a white (W) fruit developmental stage. More importantly, application of exogenous ABA to large green (LG) fruit and W fruit markedly increased endogenous ABA content, accelerated fruit ripening, and greatly enhanced the expression of FaASR transcripts and the accumulation of FaASR protein simultaneously. These results indicate that FaASR may be involved in strawberry fruit ripening. The observed increase in endogenous ABA content, and enhanced FaASR expression at transcriptional and translational levels in response to ABA treatment might partially contribute to the acceleration of strawberry fruit ripening.
登录
查看更多内容
影响因子：
6.9
作者：
Giribaldi M;Gény L;Delrot S;Schubert A
通讯作者：
Schubert A
影响因子：
5.2
作者：
Bustamante, Claudia A.;Civello, Pedro M.;Martinez, Gustavo A.
通讯作者：
Martinez, Gustavo A.
影响因子：
6.9
作者：
Aharoni, A;O'Connell, AP
通讯作者：
O'Connell, AP
影响因子：
7.4
作者：
Davies, C;Boss, PK;Robinson, SP
通讯作者：
Robinson, SP
影响因子：
3.5
作者：
Frankel, Nicolas;Carrari, Fernando;Iusem, Norberto D.
通讯作者：
Iusem, Norberto D.