Salmon louse (Lepeophtheirus salmonis) transcriptomes during post molting maturation and egg production, revealed using EST-sequencing and microarray analysis.
Salmon louse (Lepeophtheirus salmonis) transcriptomes during post molting maturation and egg production, revealed using EST-sequencing and microarray analysis.
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DOI：
10.1186/1471-2164-9-126
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发表时间：
2008-03-10
期刊：
影响因子：
4.4
通讯作者：
Nilsen F
中科院分区：
文献类型：
--
作者：
Eichner C;Frost P;Dysvik B;Jonassen I;Kristiansen B;Nilsen F
鲑科Lepeophtheirus salmonis是一种外寄生桡足动物，以鲑科宿主的皮肤、粘液和血液为食。对沙门氏菌成虫前期和成虫期EST序列的初步分析显示了大量的新转录本。为了将未知转录本与生物学功能联系起来，我们将EST测序和微阵列分析相结合，以表征雌性鲑鱼虱在蜕皮后成熟和产卵过程中的转录组。EST序列分析表明，43%的EST在GenBank中无显著匹配。测序后的ESTs被组装成556个contigs和1614个单子，无论何时发现同源基因，都没有明显的与任何特定动物群体的同源基因的相关性。将27种沙门氏菌蛋白与人类、斑马鱼、昆虫和甲壳类动物的同源蛋白进行序列比较，发现其序列与所有物种几乎相同。对成熟雌性成年鲑鱼虱的微阵列分析揭示了两种主要的转录模式；在最终蜕皮期间上调，在蜕皮后生长和产蛋期间下调和雌性特异性上调。第三小组est转录在从前成虫II到未成熟成虫的蜕皮过程中下降。在蜕皮过程中调节的基因通常与角质层蛋白相匹配，而在蜕皮后生长过程中上调的转录本是雌性特有的，包括两种卵黄原蛋白。桡足动物沙门氏菌含有大量的新基因。在分析的沙门氏菌蛋白中，与甲壳类动物同源蛋白的序列一致性不高于与人类同源蛋白的序列一致性。蜕皮、蜕皮后生长和产蛋三个不同的过程与三组转录物的转录调控相关；两个包括与生长有关的基因，一个包括与产蛋有关的基因。本文讨论了受调控转录本的功能与成年雌鲑虱蜕皮后形态变化的关系。有明确的证据表明，在腹部和生殖器部分蜕皮后生长发生之前，主要卵黄蛋白的转录并没有被诱导。观察到的生长的一个标志是许多假定的角质层蛋白在尺寸增加之前的转录。
Lepeophtheirus salmonis is an ectoparasitic copepod feeding on skin, mucus and blood from salmonid hosts. Initial analysis of EST sequences from pre adult and adult stages of L. salmonis revealed a large proportion of novel transcripts. In order to link unknown transcripts to biological functions we have combined EST sequencing and microarray analysis to characterize female salmon louse transcriptomes during post molting maturation and egg production. EST sequence analysis shows that 43% of the ESTs have no significant hits in GenBank. Sequenced ESTs assembled into 556 contigs and 1614 singletons and whenever homologous genes were identified no clear correlation with homologous genes from any specific animal group was evident. Sequence comparison of 27 L. salmonis proteins with homologous proteins in humans, zebrafish, insects and crustaceans revealed an almost identical sequence identity with all species. Microarray analysis of maturing female adult salmon lice revealed two major transcription patterns; up-regulation during the final molting followed by down regulation and female specific up regulation during post molting growth and egg production. For a third minor group of ESTs transcription decreased during molting from pre-adult II to immature adults. Genes regulated during molting typically gave hits with cuticula proteins whilst transcripts up regulated during post molting growth were female specific, including two vitellogenins. The copepod L.salmonis contains high a level of novel genes. Among analyzed L.salmonis proteins, sequence identities with homologous proteins in crustaceans are no higher than to homologous proteins in humans. Three distinct processes, molting, post molting growth and egg production correlate with transcriptional regulation of three groups of transcripts; two including genes related to growth, one including genes related to egg production. The function of the regulated transcripts is discussed in relation to post molting morphological changes in adult female salmon louse. There is clear evidence that transcription of the major yolk proteins is not induced before some of the post molting growth of abdomen and the genital segment has occurred. A hallmark for the observed growth is transcription of many putative cuticula proteins prior to the size increase.
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影响因子：
1.3
作者：
Basso, W;Venturini, L;Dubey, JP
通讯作者：
Dubey, JP
影响因子：
4.4
作者：
Dana AN;Hong YS;Kern MK;Hillenmeyer ME;Harker BW;Lobo NF;Hogan JR;Romans P;Collins FH
通讯作者：
Collins FH
影响因子：
64.5
作者：
BASTIANI, MJ;HARRELSON, AL;GOODMAN, CS
通讯作者：
GOODMAN, CS
影响因子：
3.5
作者：
Kvamme, BO;Kongshaug, H;Nilsen, F
通讯作者：
Nilsen, F
影响因子：
2
作者：
Grimnes, A;Jakobsen, PJ
通讯作者：
Jakobsen, PJ