Osteocalcin expression by circulating endothelial progenitor cells in patients with coronary atherosclerosis.
Osteocalcin expression by circulating endothelial progenitor cells in patients with coronary atherosclerosis.
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DOI：
10.1016/j.jacc.2008.07.019
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发表时间：
2008-10-14
影响因子：
24
通讯作者：
Khosla, Sundeep
中科院分区：
文献类型：
--
作者：
Goessl, Mario;Moedder, Ulrike I.;Atkinson, Elizabeth J.;Lerman, Amir;Khosla, Sundeep
为了测试冠状动脉粥样硬化患者循环内皮祖细胞(EPC)表达成骨表型是否增加。越来越多的证据表明，骨和血管系统之间存在联系，骨髓和循环中的成骨细胞已经通过成骨细胞标志物骨钙素(OCN)的染色来鉴定。内皮祖细胞有助于血管修复，但修复血管损伤可能导致钙化。使用细胞表面标志物(CD34、CD133、KDR)识别内皮祖细胞，我们检测冠状动脉粥样硬化患者内皮祖细胞表达OCN的百分比是否增加。我们研究了72例接受有创冠状动脉评估的患者：对照组(冠状动脉正常，无内皮功能障碍，n=21)和冠状动脉粥样硬化组：早期冠状动脉粥样硬化(ECA：冠状动脉正常但有内皮功能障碍，n=22)和晚期冠状动脉粥样硬化(LCA：严重多支冠状动脉疾病[CAD]，n=29)。用流式细胞仪分析外周血单核细胞。与对照组相比，食管瘤和肝细胞癌患者CD34+/Kdr+和CD34+/CD133+/Kdr+细胞共染色OCN的百分率显著增加(~2倍)，其中CD34+/CD133−/Kdr+细胞共染色OCN的百分比增加更多(分别为5倍和2倍，P<；0.001和0.05)。与内皮功能正常和无结构性冠状动脉病变的患者相比，冠状动脉粥样硬化患者内皮祖细胞表达OCN的比例更高。这些发现对血管钙化的机制和开发新的冠状动脉粥样硬化标记物具有潜在的意义。
To test whether patients with coronary atherosclerosis have increases in circulating endothelial progenitor cells (EPCs) expressing an osteogenic phenotype. Increasing evidence indicates a link between bone and the vasculature, and bone marrow and circulating osteogenic cells have been identified by staining for the osteoblastic marker, osteocalcin (OCN). EPCs contribute to vascular repair, but repair of vascular injury may result in calcification. Using cell surface markers (CD34, CD133, KDR) to identify EPCs, we examined whether patients with coronary atherosclerosis had increases in the percentage of EPCs expressing OCN. We studied 72 patients undergoing invasive coronary assessment: Controls (normal coronary arteries and no endothelial dysfunction, n = 21) versus two groups with coronary atherosclerosis: early coronary atherosclerosis (ECA: normal coronary arteries but with endothelial dysfunction, n = 22) and late coronary atherosclerosis (LCA: severe, multi-vessel coronary artery disease [CAD], n = 29). Peripheral blood mononuclear cells were analyzed using flow cytometry. Compared to controls, patients with ECA or LCA had significant increases (~2-fold) in the percentage of CD34+/KDR+ and CD34+/CD133+/KDR+ cells co-staining for OCN; even larger increases were noted in the ECA and LCA patients in the percentage of CD34+/CD133−/KDR+ cells co-staining for OCN (5- and 2-fold, P < 0.001 and 0.05, respectively). A higher percentage of EPCs express OCN in patients with coronary atherosclerosis compared with subjects with normal endothelial function and no structural CAD. These findings have potential implications for the mechanisms of vascular calcification and for the development of novel markers for coronary atherosclerosis.
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影响因子：
20.1
作者：
Friedrich, EB;Walenta, K;Werner, N
通讯作者：
Werner, N
影响因子：
5.2
作者：
Chen, JL;Hunt, P;Choi, ESH
通讯作者：
Choi, ESH
影响因子：
158.5
作者：
Eghbali-Fatourechi, GZ;Lamsam, J;Khosla, S
通讯作者：
Khosla, S
影响因子：
2.1
作者：
Gabbasov, Zufar A.;Agapov, Alexander A.;Smirnov, Vladimir N.
通讯作者：
Smirnov, Vladimir N.
影响因子：
120.7
作者：
Holmes, DR;Teirstein, P;Cohen, SA
通讯作者：
Cohen, SA