Comparative genomic, proteomic and exoproteomic analyses of three Pseudomonas strains reveals novel insights into the phosphorus scavenging capabilities of soil bacteria.
Comparative genomic, proteomic and exoproteomic analyses of three Pseudomonas strains reveals novel insights into the phosphorus scavenging capabilities of soil bacteria.
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DOI：
10.1111/1462-2920.13390
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发表时间：
2016-10
影响因子：
5.1
通讯作者：
Wellington EM
中科院分区：
文献类型：
--
作者：
Lidbury ID;Murphy AR;Scanlan DJ;Bending GD;Jones AM;Moore JD;Goodall A;Hammond JP;Wellington EM
栖息在农作物根际的细菌可以对作物生长产生有益的影响。一种这样的机制是微生物驱动的磷（P）的复合形式的溶解和再矿化。已知细菌响应于低P条件而分泌各种磷酸酶。然而，我们的理解，他们的全球蛋白质组响应磷胁迫是有限的。在这里，恶臭假单胞菌BIRD-1（BIRD-1），荧光假单胞菌SBW 25和施氏假单胞菌DSM 4166的外蛋白质组学分析与在磷酸盐（Pi）充足和Pi耗尽条件下生长的BIRD-1的全细胞蛋白质组学分析一致进行。比较外蛋白质组学揭示了响应于Pi耗尽的每个假单胞菌菌株的外蛋白质组的显著异质性。除了PHO调节子的良好表征的成员（如碱性磷酸酶）之外，还鉴定了先前与对Pi耗尽的响应无关的几种蛋白质。这些包括推定的核酸酶，磷酸三酯酶，推定的膦酸盐转运蛋白和外膜蛋白。此外，在BIRD-1中，主调节因子phoBR的诱变使我们确认了几种新的PHO依赖性蛋白的添加。我们的数据扩展了假单胞菌PHO调节子的知识，包括经常用作生物接种剂的物种，为更有效和更完整地利用土壤复合磷开辟了潜力。
Bacteria that inhabit the rhizosphere of agricultural crops can have a beneficial effect on crop growth. One such mechanism is the microbial‐driven solubilization and remineralization of complex forms of phosphorus (P). It is known that bacteria secrete various phosphatases in response to low P conditions. However, our understanding of their global proteomic response to P stress is limited. Here, exoproteomic analysis of Pseudomonas putida BIRD‐1 (BIRD‐1), Pseudomonas fluorescens SBW25 and Pseudomonas stutzeri DSM4166 was performed in unison with whole‐cell proteomic analysis of BIRD‐1 grown under phosphate (Pi) replete and Pi deplete conditions. Comparative exoproteomics revealed marked heterogeneity in the exoproteomes of each Pseudomonas strain in response to Pi depletion. In addition to well‐characterized members of the PHO regulon such as alkaline phosphatases, several proteins, previously not associated with the response to Pi depletion, were also identified. These included putative nucleases, phosphotriesterases, putative phosphonate transporters and outer membrane proteins. Moreover, in BIRD‐1, mutagenesis of the master regulator, phoBR, led us to confirm the addition of several novel PHO‐dependent proteins. Our data expands knowledge of the Pseudomonas PHO regulon, including species that are frequently used as bioinoculants, opening up the potential for more efficient and complete use of soil complexed P.
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影响因子：
4.8
作者：
Borisova, Svetlana A.;Christman, Harry D.;Metcalf, William W.
通讯作者：
Metcalf, William W.
影响因子：
2.1
作者：
Baek, Jong Hwan;Lee, Sang Yup
通讯作者：
Lee, Sang Yup
影响因子：
3.6
作者：
Ball, G;Durand, É;Filloux, A
通讯作者：
Filloux, A
影响因子：
3.2
作者：
JIANG, WH;METCALF, WW;WANNER, BL
通讯作者：
WANNER, BL
影响因子：
5.1
作者：
Christie-Oleza JA;Armengaud J;Guerin P;Scanlan DJ
通讯作者：
Scanlan DJ