Antimicrobial Susceptibility of Staphylococcus aureus Isolated from Recreational Waters and Beach Sand in Eastern Cape Province of South Africa.
Antimicrobial Susceptibility of Staphylococcus aureus Isolated from Recreational Waters and Beach Sand in Eastern Cape Province of South Africa.
复制标题
DOI：
10.3390/ijerph14091001
复制
发表时间：
2017-09-01
影响因子：
--
通讯作者：
Clarke AM
中科院分区：
文献类型：
--
作者：
Akanbi OE;Njom HA;Fri J;Otigbu AC;Clarke AM
背景资料：金黄色葡萄球菌对常用抗生素的耐药性与其在自然界中获得和传播抗生素耐药性决定因素的能力有关，海洋环境可能成为耐药性细菌的储存库。本研究确定了S.金黄色葡萄球菌分离自南非东开普省的选定海滩水和潮间带海滩沙。方法：于2015年4月至2016年4月在10个海滩采集249个海滩沙和水样品。使用标准微生物学方法从样品中分离金黄色葡萄球菌，并进行15种抗生素的敏感性试验。耐甲氧西林金黄色葡萄球菌（MRSA）通过对苯唑西林的敏感性和在Brilliance MRSA II琼脂上的生长来检测。筛选出mecA、femA、rpoB、blaZ、ermB、ermA、ermC、vanA、vanB、tetK和tetM等耐药基因。结果：30株（12.3%）为S.金黄色葡萄球菌的PCR检测结果显示，超过50%的金黄色葡萄球菌对甲氧西林表现出表型耐药。S的抗性。金黄色葡萄球菌对抗生素的耐药性差异很大，最高耐药记录为氨苄西林和青霉素（96.7%），利福平和克林霉素（80%），苯唑西林（73.3%）和红霉素（70%）。S.金黄色葡萄球菌对亚胺培南（96.7%）、左氧氟沙星（86.7%）、氯霉素（83.3%）、头孢西丁（76.7%）、环丙沙星（66.7%）、庆大霉素（63.3%）、四环素和磺胺甲恶唑-甲氧苄啶（56.7%）、万古霉素和强力霉素（50%）敏感。所有30例（100%）S。金黄色葡萄球菌分离株显示出多种抗生素耐药模式（对三种或更多种抗生素耐药）。5例患者检测到mecA、femA、rpoB、blaZ、ermB和tetM基因（22.7%）、16（53.3%），11个（45.8%）、16（55.2%）、15（71.4%），8（72.7%）;结论：研究结果表明，南非东开普省的海滩水和砂可能是耐南极洲的潜在储集层。金黄色葡萄球菌，可传播给暴露的人和动物。
Background: Resistance of Staphylococcus aureus to commonly used antibiotics is linked to their ability to acquire and disseminate antimicrobial-resistant determinants in nature, and the marine environment may serve as a reservoir for antibiotic-resistant bacteria. This study determined the antibiotic sensitivity profile of S. aureus isolated from selected beach water and intertidal beach sand in the Eastern Cape Province of South Africa. Methods: Two hundred and forty-nine beach sand and water samples were obtained from 10 beaches from April 2015 to April 2016. Staphylococcus aureus was isolated from the samples using standard microbiological methods and subjected to susceptibility testing to 15 antibiotics. Methicillin-resistant Staphylococcus aureus (MRSA) was detected by susceptibility to oxacillin and growth on Brilliance MRSA II agar. Antibiotic resistance genes including mecA, femA rpoB, blaZ, ermB, ermA, ermC, vanA, vanB, tetK and tetM were screened. Results: Thirty isolates (12.3%) were positive for S. aureus by PCR with over 50% showing phenotypic resistance to methicillin. Resistance of S. aureus to antibiotics varied considerably with the highest resistance recorded to ampicillin and penicillin (96.7%), rifampicin and clindamycin (80%), oxacillin (73.3%) and erythromycin (70%). S. aureus revealed varying susceptibility to imipenem (96.7%), levofloxacin (86.7%), chloramphenicol (83.3%), cefoxitin (76.7%), ciprofloxacin (66.7%), gentamycin (63.3%), tetracycline and sulfamethoxazole-trimethoprim (56.7%), and vancomycin and doxycycline (50%). All 30 (100%) S. aureus isolates showed multiple antibiotic-resistant patterns (resistant to three or more antibiotics). The mecA, femA, rpoB, blaZ, ermB and tetM genes were detected in 5 (22.7%), 16 (53.3%), 11 (45.8%), 16 (55.2%), 15 (71.4%), and 8 (72.7%) isolates respectively; Conclusions: Results from this study indicate that beach water and sand from the Eastern Cape Province of South Africa may be potential reservoirs of antibiotic-resistant S. aureus which could be transmitted to exposed humans and animals.
登录
查看更多内容
影响因子：
2.7
作者：
CHAROENCA, N;FUJIOKA, RS
通讯作者：
FUJIOKA, RS
影响因子：
2.4
作者：
Ateba, CN;Mbewe, M;Bezuidenhout, CC
通讯作者：
Bezuidenhout, CC
影响因子：
12.8
作者：
Borjesson, Stefan;Melin, Sara;Lindgren, Per-Eric
通讯作者：
Lindgren, Per-Eric
影响因子：
12.8
作者：
Elmir, Samir M.;Wright, Mary E.;Quaye, Elesi A.
通讯作者：
Quaye, Elesi A.
DOI：
10.1080/09603129109356701
发表时间：
1991-03-01
影响因子：
3.2
作者：
Calderon, R L;Mood, E W;Dufour, A P
通讯作者：
Dufour, A P