A microfluidic device for motility and osmolality analysis of zebrafish sperm.
A microfluidic device for motility and osmolality analysis of zebrafish sperm.
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DOI：
10.1007/s10544-018-0308-2
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发表时间：
2018-08-09
影响因子：
2.8
通讯作者：
Todd Monroe W
中科院分区：
文献类型：
--
作者：
Beckham J;Alam F;Omojola V;Scherr T;Guitreau A;Melvin A;Park DS;Choi JW;Tiersch TR;Todd Monroe W
描述了一种微流控芯片，其有助于斑马鱼精子的研究和质量控制分析，由于其微小（即，2-5μl）样品体积和短的运动持续时间（即，<1分钟），对传统的手动评估方法提出了挑战。使用结合到玻璃基底的聚二甲基硅氧烷（PDMS）中模制的微混合器通过与水混合来激活精子样品，通过用户按压连接到芯片的移液管来启动。微流控观察室中的样品流能够在1秒内停止，从而允许使用已建立的计算机辅助精子分析（CASA）方法对样品进行快速分析。斑马鱼精子细胞活化与手动混合一致，并在较早的时间点产生更高的活力值，以及更微妙的时间依赖性的运动趋势，比那些用手处理。精子激活曲线，这表明样品质量通过评估百分比和持续时间的运动在不同的解决方案渗透压，产生与芯片上的微加工金地板电极询问阻抗谱。导纳的大小与渗透压摩尔浓度成线性比例，并且不受电极附近精子细胞的存在的影响。该设备代表了简化方法的关键一步，用于一致，快速评估水生物种的精子质量。使用本文所述的微流体装置快速激活精子并用CASA一致地测量运动性的能力将有助于提高对精子的研究的再现性并有助于种质库的开发。
microfluidic chip is described that facilitates research and quality control analysis of zebrafish sperm which, due to its miniscule (i.e., 2–5 μl) sample volume and short duration of motility (i.e., <1 min), present a challenge for traditional manual assessment methods. A micromixer molded in polydimethylsiloxane (PDMS) bonded to a glass substrate was used to activate sperm samples by mixing with water, initiated by the user depressing a transfer pipette connected to the chip. Sample flow in the microfluidic viewing chamber was able to be halted within 1 s, allowing for rapid analysis of the sample using established computer-assisted sperm analysis (CASA) methods. Zebrafish sperm cell activation was consistent with manual hand mixing and yielded higher values of motility at earlier time points, as well as more subtle time-dependent trends in motility, than those processed by hand. Sperm activation curves, which indicate sample quality by evaluating percentage and duration of motility at various solution osmolalities, were generated with on-chip microfabricated gold floor electrodes interrogated by impedance spectroscopy. The magnitude of admittance was linearly proportional to osmolality and was not affected by the presence of sperm cells in the vicinity of the electrodes. This device represents a pivotal step in streamlining methods for consistent, rapid assessment of sperm quality for aquatic species. The capability to rapidly activate sperm and consistently measure motility with CASA using the microfluidic device described herein will help improve the reproducibility of studies on sperm and assist development of germplasm repositories.
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影响因子：
2.8
作者：
Scherr T;Knapp GL;Guitreau A;Park DS;Tiersch T;Nandakumar K;Monroe WT
通讯作者：
Monroe WT
影响因子：
2.8
作者：
Chen, Yu-An;Huang, Zi-Wei;Wo, Andrew M.
通讯作者：
Wo, Andrew M.
影响因子：
4.2
作者：
Chen, Chang-Yu;Chiang, Tsun-Chao;Wo, Andrew M.
通讯作者：
Wo, Andrew M.
挤压芯片：手指控制的微流体网络装置及其在生化分析中的应用
影响因子：
6.1
作者：
Li, Wentao;Chen, Tao;Huang, Yanyi
通讯作者：
Huang, Yanyi
影响因子：
4
作者：
Dooley, K;Zon, LI
通讯作者：
Zon, LI