Microfluidics and numerical simulation as methods for standardization of zebrafish sperm cell activation.
Microfluidics and numerical simulation as methods for standardization of zebrafish sperm cell activation.
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DOI：
10.1007/s10544-015-9957-6
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发表时间：
2015
影响因子：
2.8
通讯作者：
Monroe WT
中科院分区：
文献类型：
--
作者：
Scherr T;Knapp GL;Guitreau A;Park DS;Tiersch T;Nandakumar K;Monroe WT
精子细胞活化在从受精到冷冻保存方案评估的一系列生物和工程过程中起着关键作用。在一系列物种中，已发现导致活化的离子和渗透效应。斑马鱼（Danio rerio）精子细胞在低渗环境中启动运动。在这项研究中，我们采用了一种微流体混合器的目的是迅速稀释细胞外介质开始发病的细胞运动。微通道的使用在整个装置中提供了快速且可再现的混合分布。相对于目前的分析方法，这大大减少了试验之间的变异性。将这些实验与数值模拟相结合，我们能够研究细胞内渗透压的动态，因为每个细胞沿着其路径移动通过微混合器。我们的研究结果表明，细胞内渗透压，因此细胞内离子浓度，只有轻微的下降，相反的共同认为，这些参数的较大变化是激活所必需的。利用这一框架，微流体控制的细胞外环境和相关的数值模拟，具有实用性的标准化高通量的水生精子激活，更根本的是，导致运动的细胞内环境的调查。
Sperm cell activation plays a critical role in a range of biological and engineering processes, from fertilization to cryopreservation protocol evaluation. Across a range of species, ionic and osmotic effects have been discovered that lead to activation. Sperm cells of zebrafish (Danio rerio) initiate motility in a hypoosmotic environment. In this study, we employ a microfluidic mixer for the purpose of rapidly diluting the extracellular medium to initiate the onset of cell motility. The use of a microchannel offers a rapid and reproducible mixing profile throughout the device. This greatly reduces variability from trial to trial relative to the current methods of analysis. Coupling these experiments with numerical simulations, we were able to investigate the dynamics of intracellular osmolality as each cell moves along its path through the micromixer. Our results suggest that intracellular osmolality, and hence intracellular ion concentration, only slightly decreases, contrary to the common thought that larger changes in these parameters are required for activation. Utilizing this framework, microfluidics for controlled extracellular environments and associated numerical modeling, has practical applicability in standardizing high-throughput aquatic sperm activation, and more fundamentally, investigations of the intracellular environment leading to motility.
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DOI：
10.1085/jgp.45.1.143
发表时间：
1961-09
期刊：
The Journal of general physiology
影响因子：
--
作者：
KEDEM O;KATCHALSKY A
通讯作者：
KATCHALSKY A
影响因子：
56.9
作者：
MORISAWA, M;SUZUKI, K
通讯作者：
SUZUKI, K
影响因子：
2.8
作者：
Verstegen, J;Iguer-Ouada, M;Onclin, K
通讯作者：
Onclin, K
影响因子：
2.8
作者：
Seo, Duck-bong;Agca, Yuksel;Critser, John K.
通讯作者：
Critser, John K.
影响因子：
--
作者：
Lucké, B;Hartline, HK;Ricca, RA
通讯作者：
Ricca, RA