Six month tracking of microbial growth in a metalworking fluid after system cleaning and recharging.
Six month tracking of microbial growth in a metalworking fluid after system cleaning and recharging.
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系统清洁和充电后六个月跟踪金属加工液中的微生物生长。
DOI：
10.1093/annhyg/meh043
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Duchaine,Caroline
中科院分区：
文献类型：
--
作者：
Veillette,Marc;Thorne,PeterS;Gordon,Terry;Duchaine,Caroline
大量的金属加工液（MWF）用于制造业，用于在加工过程中冷却和润滑金属件和工具。MWF通过连续的再循环和再利用积累微生物生长。我们研究了6个月后倾倒，清洁和充电（DCR）的大型半合成MWF系统与几种杀生物剂管理的微生物污染的进展。在广泛清洁后，将新鲜的未污染流体添加到系统中。采集并分析了以下样品：DCR前液体（系统清洁前）;用水稀释至6%的纯液体; DCR后12 h和1、3和6个月的使用中MWF。通过使用荧光显微镜直接计数和在各种培养基（R2 A、BHI、Middlebrooks和虎红，需氧条件下）上平板计数，分析样品的总微生物浓度。此外，PCR用于检测分枝杆菌。通过荧光显微镜测定，细菌总数迅速增加：DCR前使用的液体中为5.7 × 107个细胞/ml，纯液体中无可测量的细菌，12 h后为6.9 × 106个细胞/ml，1、3和6个月后分别为2.2 × 106、3.6 × 108和6.1 × 108个细胞/ml。平均而言，在R2 A培养物上仅定量了0.2%的直接计数微生物。PCR显示在3个月和6个月时所用MWF中存在分枝杆菌。还从Middlebrooks和R2 A上的培养物中鉴定出分枝杆菌。本研究表明，清洁MWF系统的标准方法是不够的，因为系统中的残留细菌可以迅速重新填充新充电的MWF。
Large volumes of metalworking fluids (MWFs) are used in manufacturing industries for cooling and lubrication of metal pieces and tools during machining. MWFs accumulate microbial growth through continuous recirculation and reuse. We studied the progression of microbial contamination for 6 months after dumping, cleaning and recharging (DCR) of a large semi-synthetic MWF system managed with several biocides. Fresh, uncontaminated fluid was added to the system after extensive cleaning. The following samples were collected and analyzed: pre-DCR fluid (before system cleaning); neat fluid diluted to 6% with water; in use MWF 12 h and 1, 3 and 6 months post-DCR. Samples were analyzed for total microorganism concentrations by direct counting using fluorescence microscopy and by plate counting on various media (R2A, BHI, Middlebrooks and rose bengal under aerobic conditions). In addition, PCR was performed for the detection of mycobacteria. There was a rapid progression in the total bacterial counts as determined by fluorescence microscopy: 5·7 × 107cells/ml in the pre-DCR used fluid, no measurable bacteria in the neat fluid, 6·9 × 106cells/ml after 12 h and 2·2 × 106, 3·6 × 108and 6·1 × 108cells/ml after 1, 3 and 6 months, respectively. On average, only 0·2% of the direct count organisms were quantified on R2A cultures. PCR showed the presence of mycobacteria in the used MWF at 3 and 6 months. Mycobacteria were also identified from cultures on Middlebrooks and R2A. This study demonstrates that standard methods for cleaning MWF systems are inadequate since residual bacteria in the system can rapidly repopulate the newly charged MWF.
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用于研究急性呼吸系统影响的金属加工液暴露指数的表征。
DOI：
--
发表时间：
2000
期刊：
Applied Occupational and Environmental Hygiene
影响因子：
--
作者：
L. Abrams;N. Seixas;T. Robins;H. Burge;M. Muilenberg;A. Franzblau
通讯作者：
A. Franzblau
影响因子：
13.6
作者：
CHAZAL, PM
通讯作者：
CHAZAL, PM
影响因子：
4.4
作者：
CARSON, LA;BLAND, LA;GOOD, RC
通讯作者：
GOOD, RC
影响因子：
4.4
作者：
CARSON, LA;PETERSEN, NJ;AGUERO, SM
通讯作者：
AGUERO, SM
工业金属加工液中的微生物生长和积累
影响因子：
4.4
作者：
Inger Mattsby;Michael Sandin;Britta Ahlstrom;M. Edebo;E. Falsen;Karsten Pedersen;Nils Rodin
通讯作者：
Nils Rodin