Human monocyte-derived dendritic cells induce naive T cell differentiation into T helper cell type 2 (Th2) or Th1/Th2 effectors. Role of stimulator/responder ratio.
Human monocyte-derived dendritic cells induce naive T cell differentiation into T helper cell type 2 (Th2) or Th1/Th2 effectors. Role of stimulator/responder ratio.
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DOI：
10.1084/jem.192.3.405
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发表时间：
2000-08-07
期刊：
影响因子：
--
通讯作者：
Sarfati M
中科院分区：
文献类型：
--
作者：
Tanaka H;Demeure CE;Rubio M;Delespesse G;Sarfati M
树突状细胞(DC)的亚群和诱导DC成熟的信号的性质决定了DC产生极化免疫反应的能力。在这项研究中，我们发现人类单核细胞来源的DC(髓系DC1)促进T辅助细胞1型(Th1)或Th2分化的能力也被发现与刺激/应答比密切相关。在低比例(1：30 0)条件下，经炎症性刺激(金黄色葡萄球菌或脂多糖)或T细胞依赖(CD40L)刺激后分化的成熟DC可诱导幼稚T细胞成为Th2型(IL-4+、IL-5+、干扰素γ)效应细胞。Th2的分化依赖于B7-CD28共刺激，并被OX40-OX40配体相互作用所促进。然而，高DC/T细胞比率(1：4)有利于Th1/Th2混合细胞的发育。因此，相同的DC谱系刺激极化的Th1或Th2反应的事实可能是相关的，因为它允许抗原提呈细胞对外围部位接收到的信号启动适当的反应。控制DC向淋巴组织中T细胞区迁移的数量和速率可能对DC的治疗应用具有重要意义。
The subset of dendritic cells (DCs) and the nature of the signal inducing DC maturation determine the capacity of DCs to generate polarized immune responses. In this study, we show that the ability of human monocyte-derived DCs (myeloid DC1) to promote T helper type 1 (Th1) or Th2 differentiation was also found to be critically dependent on stimulator/responder ratio. At a low ratio (1:300), mature DCs that have been differentiated after inflammatory (Staphylococcus aureus Cowan 1 or lipopolysaccharide) or T cell–dependent (CD40 ligand) stimulation induced naive T cells to become Th2 (interleukin [IL]-4+, IL-5+, interferon γ) effectors. Th2 differentiation was dependent on B7–CD28 costimulation and enhanced by OX40–OX40 ligand interactions. However, high DC/T cell ratio (1:4) favored a mixed Th1/Th2 cell development. Thus, the fact that the same DC lineage stimulates polarized Th1 or Th2 responses may be relevant since it allows the antigen-presenting cells to initiate an appropriate response for the signal received at the peripheral sites. Controlling the number and the rate of DC migration to the T cell areas in lymphoid tissues may be important for the therapeutic use of DCs.
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DOI：
10.1084/jem.182.5.1591
发表时间：
1995-11-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Constant S;Pfeiffer C;Woodard A;Pasqualini T;Bottomly K
通讯作者：
Bottomly K
DOI：
10.1084/jem.182.5.1357
发表时间：
1995-11-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Openshaw P;Murphy EE;Hosken NA;Maino V;Davis K;Murphy K;O'Garra A
通讯作者：
O'Garra A
影响因子：
56.9
作者：
Rissoan, MC;Soumelis, V;Liu, YJ
通讯作者：
Liu, YJ
影响因子：
56.9
作者：
Randolph, GJ;Beaulieu, S;Muller, WA
通讯作者：
Muller, WA
影响因子：
5.4
作者：
KING, CL;STUPI, RJ;THYPHRONITIS, G
通讯作者：
THYPHRONITIS, G