Material Properties and Cell Compatibility of Photo-Crosslinked Sericin Urethane Methacryloyl Hydrogel.
Material Properties and Cell Compatibility of Photo-Crosslinked Sericin Urethane Methacryloyl Hydrogel.
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DOI：
10.3390/gels8090543
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发表时间：
2022-08-29
期刊：
影响因子：
--
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中科院分区：
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--
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在再生医学中，需要开发新的细胞相容性水凝胶用于细胞封装和递送。本研究的目的是合成异氰酸酯乙基甲基丙烯酰功能化丝胶，并确定其作为一种天然水凝胶的材料性能，用于再生医学中人间充质干细胞（MSCs）的包封和递送。从蚕茧中提取丝胶蛋白，分别与2-异氰基甲基丙烯酸乙酯（IEM）和甲基丙烯酸酐（MA）反应，制备丝胶蛋白氨基甲基丙烯酸酯（SerAte-UM）和丝胶蛋白甲基丙烯酸酯（SerAte-M，对照）生物聚合物。生物聚合物在水溶液中光交联产生的水凝胶具有凝胶动力学、微观结构、压缩模量、含水量、降解性、渗透性和被封装细胞的活力等特征。柠檬酸提取丝胶的二级结构不受IEM和MA功能化的影响。SerAte-UM水凝胶的亲水性略高于SerAte-M。随着改性程度的增加，SerAte-UM水凝胶的凝胶时间缩短。光聚合的SerAte-UM水凝胶具有高度多孔、纤维状、蜂窝状的微观结构，平均孔径在40-50µm范围内。seret - um水凝胶的压缩模量、溶胀比和渗透性与丝胶的改性程度有关，在水溶液中培养21天后的质量损失<25%。SerAte-UM和SerAte-M水凝胶均支持包封MSCs的生存和生长。与SerAte-M相比，SerAte-UM水凝胶具有更高的亲水性，有望作为组织工程中干细胞包封和递送的基质。
There is a need to develop novel cytocompatible hydrogels for cell encapsulation and delivery in regenerative medicine. The objective of this work was to synthesize isocyanato ethyl methacryloyl-functionalized sericin and determine its material properties as a natural hydrogel for the encapsulation and delivery of human mesenchymal stem cells (MSCs) in regenerative medicine. Sericin extracted from silk cocoons was reacted with 2-isocyanatoethyl methacrylate (IEM) or methacrylic anhydride (MA) to produce sericin urethane methacryloyl (SerAte-UM) or sericin methacryloyl (SerAte-M, control) biopolymers, respectively. The hydrogels produced by photo-crosslinking of the biopolymers in an aqueous solution were characterized with respect to gelation kinetics, microstructure, compressive modulus, water content, degradation, permeability, and viability of encapsulated cells. The secondary structure of citric acid-extracted sericin was not affected by functionalization with IEM or MA. SerAte-UM hydrogel was slightly more hydrophilic than SerAte-M. The gelation time of SerAte-UM hydrogel decreased with an increasing degree of modification. The photo-polymerized SerAte-UM hydrogel had a highly porous, fibrous, honeycomb microstructure with an average pore size in the 40–50 µm range. The compressive modulus, swelling ratio, and permeability of SerAte-UM hydrogel depended on the degree of modification of sericin, and the mass loss after 21 days of incubation in aqueous solution was <25%. Both SerAte-UM and SerAte-M hydrogels supported viability and growth in encapsulated MSCs. The SerAte-UM hydrogel, with its higher hydrophilicity compared to SerAte-M, is promising as a matrix for encapsulation and delivery of stem cells in tissue engineering.
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影响因子：
18.9
作者：
Elkhoury K;Morsink M;Sanchez-Gonzalez L;Kahn C;Tamayol A;Arab-Tehrany E
通讯作者：
Arab-Tehrany E
影响因子：
--
作者：
Kunz RI;Brancalhão RM;Ribeiro LF;Natali MR
通讯作者：
Natali MR
影响因子：
14
作者：
Lin, Ruei-Zeng;Chen, Ying-Chieh;Moreno-Luna, Rafael;Khademhosseini, Ali;Melero-Martin, Juan M.
通讯作者：
Melero-Martin, Juan M.
DOI：
10.1016/j.msec.2015.05.005
发表时间：
2015-09
期刊：
Materials science & engineering. C, Materials for biological applications
影响因子：
--
作者：
Siddiqui N;Pramanik K;Jabbari E
通讯作者：
Jabbari E
影响因子：
2.8
作者：
Aramwit, Pornanong;Damrongsakkul, Siriporn;Srichana, Teerapol
通讯作者：
Srichana, Teerapol