The jasmonic acid signaling pathway is linked to auxin homeostasis through the modulation of YUCCA8 and YUCCA9 gene expression.
The jasmonic acid signaling pathway is linked to auxin homeostasis through the modulation of YUCCA8 and YUCCA9 gene expression.
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DOI：
10.1111/tpj.12152
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发表时间：
2013-05
期刊：
影响因子：
--
通讯作者：
Pollmann S
中科院分区：
文献类型：
--
作者：
Hentrich M;Böttcher C;Düchting P;Cheng Y;Zhao Y;Berkowitz O;Masle J;Medina J;Pollmann S
植物激素之间的相互作用在调节植物生长和发育中起着重要作用，但控制激素关系的网络，如氧脂素和生长素之间的关系，才刚刚出现。在这里，我们报告的转录调控拟南芥YUCCA基因，YUC 8和YUC 9，氧化脂。类似于以前的特点YUCCA家族成员，我们表明，YUC 8和YUC 9都参与生长素的生物合成，表现为增加生长素含量和生长素依赖的表型所显示的功能获得性突变体，以及显着降低IAA水平yuc 8和yuc 8/9敲除线。通过qPCR分析和启动子-报告基因系的显微镜检查获得的基因表达数据揭示了依赖于COI 1信号转导途径的氧脂素介导的YUC 9表达调节。为了支持这些发现，所分析的yuc敲除突变体的根显示对茉莉酸甲酯（MeJA）的响应降低。yuc 8和yuc 9突变体在子叶和下胚轴中对MeJA的相似反应表明YUC 8和YUC 9在气生组织中的功能重叠，而它们在根中的功能显示出一定的特异性，这可能部分与两个基因的不同时空表达模式有关。这些结果为氧化脂素信号和生长素稳态之间的密切功能关系提供了证据。
Interactions between phytohormones play important roles in the regulation of plant growth and development, but knowledge of the networks controlling hormonal relationships, such as between oxylipins and auxins, is just emerging. Here, we report the transcriptional regulation of two Arabidopsis YUCCA genes, YUC8 and YUC9, by oxylipins. Similarly to previously characterized YUCCA family members, we show that both YUC8 and YUC9 are involved in auxin biosynthesis, as demonstrated by the increased auxin contents and auxin-dependent phenotypes displayed by gain-of-function mutants as well as the significantly decreased IAA levels in yuc8 and yuc8/9 knockout lines. Gene expression data obtained by qPCR analysis and microscopic examination of promoter-reporter lines reveal an oxylipin-mediated regulation of YUC9 expression that is dependent on the COI1 signal transduction pathway. In support of these findings, the roots of the analyzed yuc knockout mutants displayed a reduced response to methyl jasmonate (MeJA). The similar response of the yuc8 and yuc9 mutants to MeJA in cotyledons and hypocotyls suggests functional overlap of YUC8 and YUC9 in aerial tissues, while their function in roots show some specificity, likely in part related to different spatio-temporal expression patterns of the two genes. These results provide evidence for an intimate functional relationship between oxylipin signaling and auxin homeostasis.
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DOI：
10.1073/pnas.0704901104
发表时间：
2007-12-11
影响因子：
11.1
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