Pinocytosis in fibroblasts. Quantitative studies in vitro.
Pinocytosis in fibroblasts. Quantitative studies in vitro.
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DOI：
10.1083/jcb.63.3.949
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发表时间：
1974-12
影响因子：
7.8
通讯作者：
COHN, ZA
中科院分区：
文献类型：
--
作者：
STEINMAN, RM;SILVER, JM;COHN, ZA
辣根过氧化物酶（HRP）被用作一种标记物，以确定在几个成纤维细胞系正在进行的胞饮率。酶在流体相中被吸附，没有吸附到细胞表面的证据。细胞化学反应产物未在细胞表面发现，仅在胞内囊泡和颗粒内可见。摄取量与HRP的给药浓度和暴露持续时间成正比。HRP摄取率为0.0032-0.0035%的管理负荷每106个细胞每小时的所有细胞研究，有一个例外：L细胞，达到汇合后，逐渐增加其胞饮活性的2至4倍。L细胞摄取HRP后，HRP失活，半衰期为6- 8h。然后在L细胞中详细研究了胞饮作用的某些代谢要求。在2-38°C范围内，提高环境温度会增加胞饮作用。Q_（10）为2.7，活化能为17.6kcal/mol。在各种代谢抑制剂（氟化物、2-脱氧葡萄糖、叠氮化物和氰化物）存在下细胞ATP水平的研究表明，L细胞通过糖酵解和呼吸途径合成ATP。需要糖酵解抑制剂和呼吸抑制剂的组合才能将细胞ATP水平和胞饮细胞活性抑制至对照值的10-20%，而单独给药的药物仅具有部分作用。尽管有一个准确的定量测定液体和溶质的摄取，在组织培养细胞中的胞饮作用的功能仍然未知。
Horseradish peroxidase (HRP) was used as a marker to determine the rate of ongoing pinocytosis in several fibroblast cell lines. The enzyme was interiorized in the fluid phase without evidence of adsorption to the cell surface. Cytochemical reaction product was not found on the cell surface and was visualized only within intracellular vesicles and granules. Uptake was directly proportional to the administered concentration of HRP and to the duration of exposure. The rate of HRP uptake was 0.0032–0.0035% of the administered load per 106 cells per hour for all cells studied with one exception: L cells, after reaching confluence, progressively increased their pinocytic activity two- to fourfold. After uptake of HRP, L cells inactivated HRP with a half-life of 6–8 h. Certain metabolic requirements of pinocytosis were then studied in detail in L cells. Raising the environmental temperature increased pinocytosis over a range of 2–38°C. The Q10 was 2.7 and the activation energy, 17.6 kcal/mol. Studies on the levels of cellular ATP in the presence of various metabolic inhibitors (fluoride, 2-desoxyglycose, azide, and cyanide) showed that L cells synthesized ATP by both glycolytic and respiratory pathways. A combination of a glycolytic and a respiratory inhibitor was needed to depress cellular ATP levels as well as pinocytic activity to 10–20% of control values, whereas drugs administered individually had only partial effects. In spite of the availability of an accurate quantitative assay for fluid and solute uptake, the function of pinocytosis in tissue culture cells remains unknown.
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影响因子：
7.8
作者：
HEUSER, JE;REESE, TS
通讯作者：
REESE, TS
影响因子：
2
作者：
Casley-Smith, J. R.
通讯作者：
Casley-Smith, J. R.
影响因子：
3.7
作者：
BRANDT, PW
通讯作者：
BRANDT, PW
影响因子：
7.8
作者：
ABRAHAMS, SJ;HOLTZMAN, E
通讯作者：
HOLTZMAN, E
影响因子：
15.3
作者：
COHN, ZA;BENSON, B
通讯作者：
BENSON, B