The effect of food intake on gene expression in human peripheral blood.
The effect of food intake on gene expression in human peripheral blood.
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DOI：
10.1093/hmg/ddp476
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发表时间：
2010-01-01
影响因子：
3.5
通讯作者：
Schadt EE
中科院分区：
文献类型：
--
作者：
Leonardson AS;Zhu J;Chen Y;Wang K;Lamb JR;Reitman M;Emilsson V;Schadt EE
在血液和其他组织中，人类基因表达特征已被证明依赖于性别、年龄和一天中的时间。然而，其他可能影响基因表达的因素尚未被系统地探讨。例如，在将血液基因表达与肥胖相关特征联系起来的研究中，究竟是禁食状态还是进食状态提供的信息最多，这是一个悬而未决的问题。在这里，我们采用双臂交叉设计对人类外周血中的基因表达进行全基因组调查，以明确解决这类问题。我们能够区分个体和时间特异性影响导致的表达变化与食物摄入导致的表达变化。通过构建一个基于基因表达特征的分类器，我们证明了对食物摄入的转录反应是稳健的，该分类器将个体分类为禁食或进食状态，准确率为b> 90%。最能区分禁食和进食状态的基因表达特征更具遗传性，并且与代谢特征相关的途径具有更大的一致性。在共表达网络的背景下，探讨了基因表达性状之间的连通性结构。在禁食状态和进食状态之间观察到连接结构的变化。我们证明了差异表达和差异连通性是表征禁食和进食状态之间变化的两种互补方式。两组基因中与肥胖相关性状相关的基因都显著富集。我们的研究结果表明，这对空腹/空腹血液表达谱提供了关于个体代谢状态的更全面的信息。
Human gene expression traits have been shown to be dependent on gender, age and time of day in blood and other tissues. However, other factors that may impact gene expression have not been systematically explored. For example, in studies linking blood gene expression to obesity related traits, whether the fasted or fed state will be the most informative is an open question. Here, we employed a two-arm cross-over design to perform a genome-wide survey of gene expression in human peripheral blood to address explicitly this type of question. We were able to distinguish expression changes due to individual and time-specific effects from those due to food intake. We demonstrate that the transcriptional response to food intake is robust by constructing a classifier from the gene expression traits with >90% accuracy classifying individuals as being in the fasted or fed state. Gene expression traits that were best able to discriminate the fasted and fed states were more heritable and achieved greater coherence with respect to pathways associated with metabolic traits. The connectivity structure among gene expression traits was explored in the context of coexpression networks. Changes in the connectivity structure were observed between the fasted and fed states. We demonstrate that differential expression and differential connectivity are two complementary ways to characterize changes between fasted and fed states. Both gene sets were significantly enriched for genes associated with obesity related traits. Our results suggest that the pair of fasted/fed blood expression profiles provide more comprehensive information about an individual's metabolic states.
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影响因子：
56.9
作者：
Pinto, S;Roseberry, AG;Horvath, TL
通讯作者：
Horvath, TL
影响因子：
64.8
作者：
Hartwell, LH;Hopfield, JJ;Murray, AW
通讯作者：
Murray, AW
影响因子：
2.8
作者：
Bourin, P;Ledain, AF;Lévi, F
通讯作者：
Lévi, F
DOI：
10.1038/oby.2005.61
发表时间：
2005-03-01
期刊：
OBESITY RESEARCH
影响因子：
--
作者：
Heilbronn, LK;Civitarese, AE;Ravussin, E
通讯作者：
Ravussin, E
影响因子：
4.7
作者：
Lum, PY;Chen, YQ;Schadt, EE
通讯作者：
Schadt, EE