Mapping neurogenesis onset in the optic tectum of Xenopus laevis.
Mapping neurogenesis onset in the optic tectum of Xenopus laevis.
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DOI：
10.1002/dneu.22393
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发表时间：
2016-12
影响因子：
3
通讯作者：
Akerman, Colin J.
中科院分区：
文献类型：
--
作者：
Herrgen, Leah;Akerman, Colin J.
神经祖细胞在脊椎动物大脑的发育和进化中起着重要作用。在早期大脑发育过程中，神经祖细胞首先通过反复增殖分裂扩大数量，然后开始出现神经原性分裂。非洲爪蟾透明且实验可及的视神经顶盖是研究神经发生细胞生物学的良好模型系统，但该系统尚未探讨增生性祖细胞与神经发生祖细胞之间的精确时空关系。在这里，我们通过对个体祖细胞及其后代的谱系追踪，构建了增殖和神经源性分裂的空间图。我们发现沿视神经顶盖前后轴的增生性祖细胞和神经源性祖细胞在空间上明显分离，增生性祖细胞位于更后方，神经源性祖细胞位于更前方。由于单个祖细胞在成熟时被重新定位到更前面的位置，这种空间分离可能反映了单个祖细胞增殖潜力的增加限制。然后，我们研究了从增殖行为到神经发生行为的转变是否与先前被认为与调节神经发生发生有关的细胞特性有关。我们的数据显示，从增殖到神经发生的转变与卵裂面方向的微小变化和细胞周期动力学的更明显变化有关，这让人想起哺乳动物系统的观察结果。我们的研究结果强调了利用非洲爪蟾的视神经顶盖作为神经发生细胞生物学研究的一个可接近的系统的潜力。
Neural progenitor cells have a central role in the development and evolution of the vertebrate brain. During early brain development, neural progenitors first expand their numbers through repeated proliferative divisions and then begin to exhibit neurogenic divisions. The transparent and experimentally accessible optic tectum of Xenopus laevis is an excellent model system for the study of the cell biology of neurogenesis, but the precise spatial and temporal relationship between proliferative and neurogenic progenitors has not been explored in this system. Here we construct a spatial map of proliferative and neurogenic divisions through lineage tracing of individual progenitors and their progeny. We find a clear spatial separation of proliferative and neurogenic progenitors along the anterior-posterior axis of the optic tectum, with proliferative progenitors located more posteriorly and neurogenic progenitors located more anteriorly. Since individual progenitors are repositioned toward more anterior locations as they mature, this spatial separation likely reflects an increased restriction in the proliferative potential of individual progenitors. We then examined whether the transition from proliferative to neurogenic behavior correlates with cellular properties that have previously been implicated in regulating neurogenesis onset. Our data reveal that the transition from proliferation to neurogenesis is associated with a small change in cleavage plane orientation and a more pronounced change in cell cycle kinetics in a manner reminiscent of observations from mammalian systems. Our findings highlight the potential to use the optic tectum of Xenopus laevis as an accessible system for the study of the cell biology of neurogenesis.
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影响因子：
16.2
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Huttner, WB
DOI：
10.1073/pnas.0437969100
发表时间：
2003-03-04
影响因子：
11.1
作者：
Haydar, TF;Ang, E;Rakic, P
通讯作者：
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