Imaging of lipids in rat heart by MALDI-MS with silver nanoparticles.
Imaging of lipids in rat heart by MALDI-MS with silver nanoparticles.
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DOI：
10.1007/s00216-013-7525-6
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发表时间：
2014-03
影响因子：
4.3
通讯作者：
Woods AS
中科院分区：
文献类型：
--
作者：
Jackson SN;Baldwin K;Muller L;Womack VM;Schultz JA;Balaban C;Woods AS
脂质是心脏组织的主要组成部分，并执行几个重要功能，如能量储存，信号传导，并作为生物膜的构建块。心脏脂质组相当多样化，由甘油磷脂如磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）、磷脂酰肌醇（PI）、磷脂酰甘油（PG）、心磷脂（CL）和甘油酯（主要是三酰甘油（TAG））组成。在这项研究中，质谱成像（MSI）使基质植入离子化的银纳米粒子（AgNP）被用来映射几类脂质在心脏组织。AgNP基质植入的使用是由我们先前的工作激发的，该工作表明，仅1014/cm 2的2nm金纳米颗粒植入到组织近表面的前10 nm中，使得能够比传统的有机MALDI基质更有效地检测大多数脑脂质（包括中性脂质物质，如β-内酰胺类）。在本文中，在整个心脏组织切片上类似地植入500 eV AgNP-导致快速、可再现、无溶剂、均匀的6 nm AgNP基质浓度驻留在组织表面附近。无论是正离子还是负离子的MALDI-MSI分析都能产生几种心脏脂质种类的高质量图像。在负离子模式下，对24种脂质物质[16种PE、4种PI、1种PG、1种CL、2种鞘磷脂（SM）]进行成像。还从29种脂质物质（10种PC、5种PE、5种SM、9种TAG）中获得了正离子图像，TAG物质大量集中在心脏的血管区域。
Lipids are a major component of heart tissue and perform several important functions such as energy storage, signaling, and as building blocks of biological membranes. The heart lipidome is quite diverse consisting of glycerophospholipids such as phosphatidylcholines (PCs), phosphatidylethanolamines (PEs), phosphatidylinositols (PIs), phosphatidylglycerols (PGs), cardiolipins (CLs), and glycerolipids, mainly triacylglycerols (TAGs). In this study, mass spectrometry imaging (MSI) enabled by matrix implantation of ionized silver nanoparticles (AgNP) was used to map several classes of lipids in heart tissue. The use of AgNP matrix implantation was motivated by our previous work showing that implantation doses of only 1014/cm2 of 2 nm gold nanoparticulates into the first 10 nm of the near surface of the tissue enabled detection of most brain lipids (including neutral lipid species such as cerebrosides) more efficiently than traditional organic MALDI matrices. Herein, a similar implantation of 500 eV AgNP− across the entire heart tissue section results in a quick, reproducible, solvent-free, uniform matrix concentration of 6 nm AgNP residing near the tissue surface. MALDI-MSI analysis of either positive or negative ions produce high-quality images of several heart lipid species. In negative ion mode, 24 lipid species [16 PEs, 4 PIs, 1 PG, 1 CL, 2 sphingomyelins (SMs)] were imaged. Positive ion images were also obtained from 29 lipid species (10 PCs, 5 PEs, 5 SMs, 9 TAGs) with the TAG species being heavily concentrated in vascular regions of the heart.
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影响因子：
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作者：
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DOI：
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影响因子：
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