Molecular Microscopy of Brain Gangliosides: Illustrating their Distribution in Hippocampal Cell Layers.
Molecular Microscopy of Brain Gangliosides: Illustrating their Distribution in Hippocampal Cell Layers.
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脑神经毒剂的分子显微镜：说明它们在海马细胞层中的分布。
DOI：
10.1021/cn100096h
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发表时间：
2011-02-21
影响因子：
5
通讯作者：
Woods, Amina S.
中科院分区：
文献类型：
--
作者：
Colsch, Benoit;Jackson, Shelley N.;Dutta, Sucharita;Woods, Amina S.
神经节苷脂是在所有脊椎动物细胞的质膜外层中发现的两亲性分子。它们在细胞识别和信号传导中发挥重要作用，并参与影响中枢神经系统（CNS）的疾病。我们报告的差异分布的神经节苷脂种类在大鼠大脑的大脑，基于其神经酰胺相关的核心，并首次乙酰化的存在下检测到的基质辅助激光解吸/电离（MALDI）质谱，这是用来映射和图像神经节苷脂详细的结构信息和组织学的准确性。在海马中，主要种类GM 1、GD 1、O-乙酰GD 1、GT 1和O-乙酰GT 1的定位取决于齿状回（ML）分子层中的鞘氨醇碱基（d18：1鞘氨醇或d20：1表胆氨醇），齿状回（ML）由三个不同的层组成，内分子层（IML）仅含有鞘氨醇，中分子层（MML）和外分子层（OML）中表胆氨醇是唯一的鞘氨醇碱基。这些结果表明，神经节苷脂在神经元轴突和树突中的分布不同，这取决于每层的神经酰胺核心。GM 3、GM 2、GD 3和GD 2主要含有鞘氨醇，并且主要存在于体细胞层中，体细胞层由锥体细胞层（Py）和齿状回（GL）的颗粒层组成，与GQ 1和O-乙酰化形式的GD 1、GT 1和GQ 1神经节苷脂相反，后者含有两种鞘氨醇碱。然而，它们的分布基于神经元细胞体中的唾液酸化和乙酰化寡糖链。
Gangliosides are amphiphilic molecules found in the outer layer of plasma membranes of all vertebrate cells. They play a major role in cell recognition and signaling and are involved in diseases affecting the central nervous system (CNS). We are reporting the differential distribution of ganglioside species in the rat brain’s cerebrum, based on their ceramide associated core, and for the first time the presence of acetylation detected by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, which was used to map and image gangliosides with detailed structural information and histological accuracy. In the hippocampus, localization of the major species GM1, GD1, O-acetylGD1, GT1, and O-acetylGT1 depends on the sphingoïd base (d18:1 sphingosine or d20:1 eïcosasphingosine) in the molecular layer of the dentate gyrus (ML), which is made up of three distinct layers, the inner molecular layer (IML), which contains sphingosine exclusively, and the middle molecular layer (MML) and the outer molecular layer (OML) where eïcosasphingosine is the only sphingoïd base. These results demonstrate that there is a different distribution of gangliosides in neuronal axons and dendrites depending on the ceramide core of each layer. GM3, GM2, GD3, and GD2 contain sphingosine predominantly and are mainly present in body cell layers, which are made up of the pyramidal cell layer (Py) and the granular layer of the dentate gyrus (GL), in contrast with GQ1 and the O-acetylated forms of GD1, GT1, and GQ1 gangliosides, which contain both sphingoïd bases. However their distribution is based on the sialylated and acetylated oligosaccharide chains in the neuronal cell bodies.
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DOI：
10.1016/s1044-0305(02)00351-3
发表时间：
2002-04-01
影响因子：
3.2
作者：
O'Connor, PB;Mirgorodskaya, E;Costello, CE
通讯作者：
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影响因子：
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DOI：
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发表时间：
2010-11-01
影响因子：
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影响因子：
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影响因子：
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