MicroRNAs 144, 145, and 214 are down-regulated in primary neurons responding to sciatic nerve transection
MicroRNAs 144, 145, and 214 are down-regulated in primary neurons responding to sciatic nerve transection
复制标题
MicroRNA 144、145 和 214 在响应坐骨神经横断的初级神经元中下调
DOI：
10.1016/j.brainres.2011.01.067
复制
发表时间：
2011-04
期刊：
影响因子：
2.9
通讯作者：
易西南
中科院分区：
文献类型：
--
作者：
易西南
MicroRNAs(MiRNAs)在包括神经系统在内的细胞和生物体的发育、分化、增殖、存活和肿瘤发生中发挥着重要作用。然而，miRNAs在损伤后背根神经节(DRG)初级神经元中的作用尚不清楚。在这项研究中，进行了miRNA微阵列分析，发现在单侧坐骨神经切断后，共有21个miRNAs表达下调。用定量逆转录聚合酶链式反应(qRT-PCR)进一步验证miR-144、miR-145和miR-214。此外，用锁定核酸(LNA)修饰的DNA寡核苷酸探针进行原位杂交实验证实，miR-144、miR-145和miR-214在DRG原代神经元中表达，并在坐骨神经切断后表达下调。通过进行生物信息学分析，确定了对潜在miRNA靶标的预测。使用miRNA荧光素酶报告载体验证了这些预测，Robo2和SRGAP2分别被评估为miR-145和miR-214的潜在靶标。我们还研究了miR-145在原代培养神经元中的作用，结果发现miR-145抑制轴突生长并下调Robo2的表达。本研究结果提示，DRG中的miRNAs可以通过Sit-Robo-srGAP信号通路介导损伤后的再生过程。
MicroRNAs (miRNAs) play an important role in the development, differentiation, proliferation, survival, and oncogenesis of cells and organisms including nervous system. However, the role of miRNAs in primary neurons of dorsal root ganglion (DRG) after injury was not clear. In this study, a miRNA microarray analysis was performed, and a total of 21 miRNAs were found to be down-regulated following unilateral sciatic nerve transection. The miR-144, miR-145, and miR-214 were further validated using quantitative reverse transcriptase PCR (qRT-PCR). Moreover, in situ hybridization (ISH) experiments using locked nucleic acid (LNA)-modified DNA oligonucleotide probes verified that miR-144, miR-145, and miR-214 were expressed in primary neurons of DRG and down-regulated following sciatic nerve transection. Predictions of potential miRNA targets involved were identified by performing a bioinformatics analysis. These predictions were tested using miRNA luciferase reporter vectors, with Robo2 and srGAP2 evaluated as the potential targets of miR-145 and miR-214, respectively. The role of miR-145 in cultured primary neurons was also investigated, and the result found that miR-145 miR-145 inhibited neurite growth and down-regulated Robo2 expression. Finding from this study suggested that miRNAs of DRG can mediated the course of regeneration including through Slit–Robo–srGAP signaling pathway after injury.
登录
查看更多内容
影响因子：
56.9
作者：
Lagos-Quintana, M;Rauhut, R;Tuschl, T
通讯作者：
Tuschl, T
影响因子：
5.6
作者：
Bin Shi;M. Prisco;G. Calin;Changfeng Liu;G. Russo;A. Giordano;R. Baserga
通讯作者：
Bin Shi;M. Prisco;G. Calin;Changfeng Liu;G. Russo;A. Giordano;R. Baserga
影响因子：
4.6
作者：
Chan, Lai-Sheung;Yue, Patrick Ying-Kit;Wong, Ricky Ngok-Shun
通讯作者：
Wong, Ricky Ngok-Shun
DOI：
--
发表时间：
2003-10
期刊：
Molecular cancer research : MCR
影响因子：
--
作者：
M. Michael;Susan M O' Connor;Nicholas G van Holst Pellekaan;G. Young;R. James
通讯作者：
M. Michael;Susan M O' Connor;Nicholas G van Holst Pellekaan;G. Young;R. James
影响因子：
2.6
作者：
Zhan, Mei;Miller, Chris P.;Song, Chao-Zhong
通讯作者：
Song, Chao-Zhong