Non-thermal atmospheric plasma ameliorates imiquimod-induced psoriasis-like skin inflammation in mice through inhibition of immune responses and up-regulation of PD-L1 expression.
Non-thermal atmospheric plasma ameliorates imiquimod-induced psoriasis-like skin inflammation in mice through inhibition of immune responses and up-regulation of PD-L1 expression.
复制标题
DOI：
10.1038/s41598-017-15725-7
复制
发表时间：
2017-11-14
影响因子：
4.6
通讯作者：
Kim CH
中科院分区：
文献类型：
--
作者：
Lee YS;Lee MH;Kim HJ;Won HR;Kim CH
血浆药物是一个新兴的治疗领域。有研究表明，血浆具有杀菌、促进伤口愈合和诱导肿瘤细胞凋亡的作用。然而，血浆对免疫细胞和免疫相关皮肤病的影响尚未得到很好的研究。在这项研究中，我们证明了非热大气等离子体（NTP）治疗可以抑制小鼠银屑病样皮肤炎症。与没有NTP治疗的小鼠相比，在咪喹莫特诱导的银屑病样小鼠皮肤中NTP治疗抑制了上皮细胞厚度和促炎分子表达的增加。此外，在NTP处理的小鼠淋巴结中，抑制了Th 17细胞的分化，Th 17细胞是银屑病发病机制的重要细胞类型。还证明了液体型血浆（LTP）（也称为间接血浆）在体外抑制Th 17细胞分化。其他体外实验表明，LTP抑制骨髓来源的树突状细胞活化。有趣的是，LTP增强了HaCaT细胞中的PD-L1表达，表明NTP可能通过增加PD-L1表达来抑制T细胞的不必要的过度活化。总之，这些结果表明NTP可用于治疗CD 4 + T细胞介导的自身免疫性疾病，如银屑病。
Plasma medicine is an emerging novel therapeutic field. It has been reported that plasma can kill bacteria, promote wound healing and induce apoptosis of tumor cells. However, the effects of plasma on immune cells and immune related skin diseases have not been well studied. In this study, we demonstrated that non-thermal atmospheric plasma (NTP) treatment could inhibit psoriasis-like skin inflammation in mice. NTP treatment in imiquimod-induced psoriasis-like mouse skin inhibited increases in epithelial cell thickness and expression of pro-inflammatory molecules compared to ones without the NTP treatment. In addition, differentiation of Th17 cells, an important cell type for pathogenesis of psoriasis, was inhibited in the NTP-treated mouse lymph nodes. It was also demonstrated that liquid type plasma (LTP), which is also known as indirect plasma, inhibited Th17 cell differentiation in vitro. Other in vitro experiments showed that LTP inhibited bone marrow-derived dendritic cell activation. Interestingly, LTP enhanced PD-L1 expression in HaCaT cells, suggesting that NTP may inhibit unwanted over-activation of T cells through increased PD-L1 expression. Taken together, these results suggest that NTP may be used in treatment of CD4+ T cell-mediated autoimmune diseases such as psoriasis.
登录
查看更多内容
影响因子：
4.6
作者：
Fathollah S;Mirpour S;Mansouri P;Dehpour AR;Ghoranneviss M;Rahimi N;Safaie Naraghi Z;Chalangari R;Chalangari KM
通讯作者：
Chalangari KM
影响因子：
64.8
作者：
Bettelli, E;Carrier, YJ;Kuchroo, VK
通讯作者：
Kuchroo, VK
影响因子：
3.7
作者：
Kim HR;Lee A;Choi EJ;Hong MP;Kie JH;Lim W;Lee HK;Moon BI;Seoh JY
通讯作者：
Seoh JY
影响因子：
4.6
作者：
Choi JH;Song YS;Lee HJ;Hong JW;Kim GC
通讯作者：
Kim GC
影响因子：
4.1
作者：
Kim, Chul-Ho;Bahn, Jae Hoon;Baek, Seung Joon
通讯作者：
Baek, Seung Joon