Pressure-driven DNA transport across an artificial nanotopography
Pressure-driven DNA transport across an artificial nanotopography
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压力驱动的 DNA 在人工纳米地形上的运输
DOI：
10.1088/1367-2630/11/7/075032
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发表时间：
2009
影响因子：
3.3
通讯作者：
D. Stein
中科院分区：
文献类型：
--
作者：
J. T. Del Bonis;W. Reisner;D. Stein
研究了压力驱动的DNA在狭缝状纳米通道中的传输，该通道具有由纳米孔线性阵列组成的嵌入式纳米形貌。我们使用荧光视频显微镜对单个DNA分子在纳米孔阵列中单列移动进行成像，进行连续的孔到孔跳跃。观察到不同的运输动力学取决于一个分子是否可以占据一个单一的坑，或被迫对着多个坑。我们解释这些结果在线性阵列的凹坑的聚合物链的自由能的标度理论。单坑内所含的分子预计将面临一个熵的自由能垒，并通过热激活运输坑之间跳跃。另一方面，对着多个凹坑的分子可以响应于所施加的流体流而将DNA轮廓从上游转移到下游凹坑，这降低了能垒。当尾随坑完全清空，或当领先的坑达到其容量，能量障碍预计将消失，低压，热激活的运输制度让位于高压，耗散运输制度。这些结果有助于我们理解聚合物在纳米约束环境中，并可能指导纳米级芯片实验室应用的发展。
The pressure-driven transport of DNA was studied in slit-like nanochannels with an embedded nanotopography consisting of linear arrays of nanopits. We imaged individual DNA molecules moving single-file down the nanopit array, undergoing sequential pit-to-pit hops using fluorescence video microscopy. Distinct transport dynamics were observed depending on whether a molecule could occupy a single pit, or was forced to subtend multiple pits. We interpret these results in terms of a scaling theory of the free energy of polymer chains in a linear array of pits. Molecules contained within a single pit are predicted to face an entropic free energy barrier, and to hop between pits by thermally activated transport. Molecules that subtend multiple pits, on the other hand, can transfer DNA contour from upstream to downstream pits in response to an applied fluid flow, which lowers the energy barrier. When the trailing pit completely empties, or when the leading pit reaches its capacity, the energy barrier is predicted to vanish, and the low-pressure, thermally activated transport regime gives way to a high-pressure, dissipative transport regime. These results contribute to our understanding of polymers in nanoconfined environments, and may guide the development of nanoscale lab-on-a-chip applications.
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影响因子：
56.9
作者：
Perkins, TT;Smith, DE;Chu, S
通讯作者：
Chu, S
影响因子：
7.4
作者：
Han, JY;Craighead, HG
通讯作者：
Craighead, HG
DOI：
10.1073/pnas.0503809102
发表时间：
2005-07-19
影响因子：
11.1
作者：
Riehn, R;Lu, MC;Austin, RH
通讯作者：
Austin, RH
DOI：
--
发表时间：
2004
影响因子：
11.1
作者：
J. Tegenfeldt;C. Prinz;H. Cao;S. Chou;W. Reisner;R. Riehn;Yan Mei Wang;E. Cox;J. Sturm;
通讯作者：
J. Tegenfeldt;C. Prinz;H. Cao;S. Chou;W. Reisner;R. Riehn;Yan Mei Wang;E. Cox;J. Sturm;
影响因子：
8.6
作者：
Reisner, W;Morton, KJ;Austin, RH
通讯作者：
Austin, RH