A portable chemotaxis platform for short and long term analysis.
A portable chemotaxis platform for short and long term analysis.
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DOI：
10.1371/journal.pone.0044995
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Karp JM
中科院分区：
文献类型：
--
作者：
Xu C;Poh YK;Roes I;O'Cearbhaill ED;Matthiesen ME;Mu L;Yang SY;Miranda-Nieves D;Irimia D;Karp JM
Flow-based microfluidic systems have been widely utilized for cell migration studies given their ability to generate versatile and precisely defined chemical gradients and to permit direct visualization of migrating cells. Nonetheless, the general need for bulky peripherals such as mechanical pumps and tubing and the complicated setup procedures significantly limit the widespread use of these microfluidic systems for cell migration studies. Here we present a simple method to power microfluidic devices for chemotaxis assays using the commercially available ALZET® osmotic pumps. Specifically, we developed a standalone chemotaxis platform that has the same footprint as a multiwell plate and can generate well-defined, stable chemical gradients continuously for up to 7 days. Using this platform, we validated the short-term (24 hours) and long-term (72 hours) concentration dependent PDGF-BB chemotaxis response of human bone marrow derived mesenchymal stem cells.
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DOI：
10.1083/jcb.130.3.725
发表时间：
1995-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kundra V;Anand-Apte B;Feig LA;Zetter BR
通讯作者：
Zetter BR
影响因子：
2.2
作者：
LAUFFENBURGER, DA;ZIGMOND, SH
通讯作者：
ZIGMOND, SH
影响因子：
4.3
作者：
Kark, LR;Karp, JM;Davies, JE
通讯作者：
Davies, JE
影响因子：
4
作者：
Paguirigan, Amy L.;Beebe, David J.
通讯作者：
Beebe, David J.
影响因子：
0.9
作者：
Oprea, WE;Karp, JM;Davies, JE
通讯作者：
Davies, JE