Testing of worn face mask and saliva for SARS-CoV-2.
Testing of worn face mask and saliva for SARS-CoV-2.
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DOI：
10.3389/fpubh.2023.1237512
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发表时间：
2023
影响因子：
5.2
通讯作者：
Kotanko, Peter
中科院分区：
文献类型：
--
作者：
Wang, Xiaoling;Thwin, Ohnmar;Haq, Zahin;Dong, Zijun;Tisdale, Lela;Fuentes, Lemuel Rivera;Grobe, Nadja;Kotanko, Peter
呼出的SARS-CoV-2可以在口罩上检测到。我们比较了佩戴口罩和匹配唾液样本上的SARS-CoV-2 RNA检测结果。我们在2021年12月至2022年3月期间进行了这项前瞻性、观察性、病例对照研究。病例包括30例近期确诊为COVID-19的中心血液透析患者。对照组包括13名血液透析患者和25名在研究期间和过去2个月没有COVID-19的诊所工作人员。佩戴4小时后采集一次性3层口罩，同时采集唾液样本。使用ThermoFisher COVID-19 Combo Kit（A47814）进行RT-PCR检测。口罩和唾液检测的特异性分别为99%和100%。口罩的检测灵敏度为62%，唾液为81%（p = 0.16）。与非免疫功能低下患者相比，免疫功能低下患者的中位病毒RNA脱落持续时间为11天或更长（22 vs. 11天，p = 0.06，对数秩检验）。虽然SARS-CoV-2测试戴口罩似乎不如唾液敏感，但它可能是强制戴口罩但不愿进行更具侵入性采样的个人的首选筛查方法。然而，优化的RNA提取方法和自动化程序是必要的，以提高测试的灵敏度和可扩展性。我们证实了免疫功能低下患者的病毒RNA脱落时间更长。
Exhaled SARS-CoV-2 can be detected on face masks. We compared tests for SARS-CoV-2 RNA on worn face masks and matched saliva samples. We conducted this prospective, observational, case-control study between December 2021 and March 2022. Cases comprised 30 in-center hemodialysis patients with recent COVID-19 diagnosis. Controls comprised 13 hemodialysis patients and 25 clinic staff without COVID-19 during the study period and the past 2 months. Disposable 3-layer masks were collected after being worn for 4 hours together with concurrent saliva samples. ThermoFisher COVID-19 Combo Kit (A47814) was used for RT-PCR testing. Mask and saliva testing specificities were 99% and 100%, respectively. Test sensitivity was 62% for masks, and 81% for saliva (p = 0.16). Median viral RNA shedding duration was 11 days and longer in immunocompromised versus non-immunocompromised patients (22 vs. 11 days, p = 0.06, log-rank test). While SARS-CoV-2 testing on worn masks appears to be less sensitive compared to saliva, it may be a preferred screening method for individuals who are mandated to wear masks yet averse to more invasive sampling. However, optimized RNA extraction methods and automated procedures are warranted to increase test sensitivity and scalability. We corroborated longer viral RNA shedding in immunocompromised patients.
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影响因子：
8.4
作者：
Raymenants, Joren;Duthoo, Wout;Stakenborg, Tim;Verbruggen, Bert;Verplanken, Julien;Feys, Jos;Van Duppen, Joost;Hanifa, Rabea;Marchal, Elisabeth;Lambrechts, Andy;Maes, Piet;Andre, Emmanuel;Van den Wijngaert, Nik;Peumans, Peter
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Shin, Sehyun
DOI：
10.1016/j.jinf.2021.03.018
发表时间：
2021-06
期刊：
The Journal of infection
影响因子：
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作者：
Williams CM;Pan D;Decker J;Wisniewska A;Fletcher E;Sze S;Assadi S;Haigh R;Abdulwhhab M;Bird P;Holmes CW;Al-Taie A;Saleem B;Pan J;Garton NJ;Pareek M;Barer MR
通讯作者：
Barer MR
影响因子：
6.4
作者：
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通讯作者：
Costa, Silvia F.