Structural Characterization, Antioxidant Activity, and Biomedical Application of Astragalus Polysaccharide Degradation Products
Structural Characterization, Antioxidant Activity, and Biomedical Application of Astragalus Polysaccharide Degradation Products
复制标题
黄芪多糖降解产物的结构表征、抗氧化活性及生物医学应用
DOI：
10.1155/2018/5136185
复制
发表时间：
2018-09
期刊：
影响因子：
--
通讯作者：
Jian-Ming Ouyang
中科院分区：
文献类型：
--
作者：
Jian-Min Wang;Xin-Yuan Sun;Jian-Ming Ouyang
为研究不同分子量黄芪多糖（APS）的抗氧化能力，采用过氧化氢降解初始分子量为11.03 kDa的原始黄芪多糖（APS 0），得到3种分子量分别为8.38（APS 1）、4.72（APS 2）和2.60 kDa（APS 3）的降解多糖。利用1H NMR、13 C NMR、FT-IR、GC/MS等手段对多糖的结构进行了表征。APS降解前后单糖组成变化不大。这些多糖的体外抗氧化能力（清除羟基和ABTS自由基和还原能力）和细胞内抗氧化能力（超氧化物歧化酶和丙二醛生成）与其分子量密切相关。如果APS的分子量很高或很低，都不利于它们的活性。只有中等分子量的APS 2显示出最大的抗氧化活性和修复人肾上皮细胞（HK-2）的能力。因此，APS 2可作为一种潜在的抗结石多糖药物。
To study the antioxidant capacity of Astragalus polysaccharides (APS) with different molecular weights, we used hydrogen peroxide to degrade original Astragalus polysaccharide (APS0) with an initial molecular weight of 11.03 kDa and obtained three degraded polysaccharides with molecular weights of 8.38 (APS1), 4.72 (APS2), and 2.60 kDa (APS3). The structures of these polysaccharides were characterized by 1H NMR, 13C NMR, FT-IR, and GC/MS. The degradation process did not cause significant changes in the main chain structure of APS. The monosaccharide component of APS before and after degradation was slightly changed. The antioxidant ability in vitro (removing hydroxyl and ABTS radicals and reducing ability) and in cells (superoxide dismutase and malondialdehyde generation) of these polysaccharides is closely related to their molecular weight. If the molecular weight of APS is very high or low, it is not conducive to their activity. Only APS2 with moderate molecular weight showed the greatest antioxidant activity and ability to repair human kidney epithelial (HK-2) cells. Therefore, APS2 can be used as a potential antistone polysaccharide drug.
登录
查看更多内容
三种海参多糖理化特性及抗凝血活性比较
影响因子：
5.4
作者：
Luo L;Wu M;Xu L;Lian W;Xiang J;Lu F;Gao N;Xiao C;Wang S;Zhao J
通讯作者：
Zhao J
影响因子：
4.5
作者：
Tian, F;Liu, Y;Zhao, BY
通讯作者：
Zhao, BY
影响因子：
--
作者：
Khan, SR
通讯作者：
Khan, SR
影响因子：
5.6
作者：
Im, SA;Oh, ST;Lee, CK
通讯作者：
Lee, CK
影响因子：
11.2
作者：
Sheng, Jiwen;Sun, Yanlong
通讯作者：
Sun, Yanlong