Vaginal Microbiota Is Stable throughout the Estrous Cycle in Arabian Maress.
Vaginal Microbiota Is Stable throughout the Estrous Cycle in Arabian Maress.
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阿拉伯母马的阴道微生物群在整个发情周期中保持稳定。
DOI：
10.3390/ani10112020
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发表时间：
2020-11-03
期刊：
影响因子：
--
通讯作者：
García-Roselló E
中科院分区：
文献类型：
--
作者：
Barba M;Martínez-Boví R;Quereda JJ;Mocé ML;Plaza-Dávila M;Jiménez-Trigos E;Gómez-Martín Á;González-Torres P;Carbonetto B;García-Roselló E
了解哪些细菌主导阴道微生物群及其在整个周期中的变化对于研究如何预防生殖疾病非常重要。在女性中，阴道微生物群以乳杆菌为主，但这种情况在其他动物中不会发生。对马阴道微生物群知之甚少。本研究的目的是描述卵巢周期中马阴道微生物群的动态。八个健康的成年阿拉伯人被用来表征阴道微生物群的标准微生物学和宏基因组程序。通过两种方法，乳酸杆菌的丰度< 2%，这意味着马阴道微生物群不受这些细菌的支配。优势细菌包括其他属，如卟啉单胞菌属和弯曲杆菌属等。没有发现阴道微生物群组成的变化，表明马阴道微生物群在整个卵巢周期中是稳定的。乳酸菌（LAB）主导人类阴道微生物群并抑制病原体增殖。在其他哺乳动物中，LAB并不主导阴道微生物群，但在卵巢周期中发生优势微生物的转移。本研究的目的是通过培养依赖和独立的方法表征马阴道微生物群，并描述其在发情和间情期的变化。取8只健康成年阿拉伯山羊发情期和间情期阴道拭子。对于培养依赖性处理，在哥伦比亚血琼脂（BA）和Man Rogosa Sharpe（MRS）琼脂上分离细菌。LAB仅占细菌分离株总数的2%，且与卵巢期无关。对于非培养物依赖性处理，使用Illumina Miseq扩增16 S核糖体RNA基因的V3/V4可变区并测序。阴道微生物群的多样性和组成在发情周期中没有变化。核心马阴道微生物组在门水平上由厚壁菌门、拟杆菌门、变形菌门和放线菌门组成。在属的水平上，它被定义为卟啉单胞菌属、弯曲杆菌属、神秘杆菌属、棒状杆菌属、链球菌属、梭杆菌属、未培养的Kiritimatiatiaestria和阿克曼氏菌属。乳酸菌在发情期仅占分类组成的0.18%，在间情期仅占0.37%。在发情期和间情期样品之间，观察到最丰富的门或属的相对丰度没有差异。
Knowing which bacteria dominate vaginal microbiota and its variation throughout the cycle is important to study how to prevent reproductive diseases. In women, vaginal microbiota is dominated by Lactobacillus but this does not happen in other animals. Little is known about equine vaginal microbiota. The aim of this study was to describe the dynamics of equine vaginal microbiota during the ovarian cycle. Eight healthy adult Arabian mares were used to characterize vaginal microbiota by standard microbiologic and metagenomic procedures. The abundance of Lactobacillus was < 2% by both methods, meaning that equine vaginal microbiota was not dominated by these bacteria. Dominant bacteria included other genera such as Porphyromonas and Campylobacter among others. No changes in vaginal microbiota composition were found, suggesting that equine vaginal microbiota was stable throughout the ovarian cycle. Lactic acid bacteria (LAB) dominate human vaginal microbiota and inhibit pathogen proliferation. In other mammals, LAB do not dominate vaginal microbiota, however shifts of dominant microorganisms occur during ovarian cycle. The study objectives were to characterize equine vaginal microbiota in mares by culture-dependent and independent methods and to describe its variation in estrus and diestrus. Vaginal swabs from 8 healthy adult Arabian mares were obtained in estrus and diestrus. For culture-dependent processing, bacteria were isolated on Columbia blood agar (BA) and Man Rogosa Sharpe (MRS) agar. LAB comprised only 2% of total bacterial isolates and were not related to ovarian phases. For culture-independent processing, V3/V4 variable regions of the 16S ribosomal RNA gene were amplified and sequenced using Illumina Miseq. The diversity and composition of the vaginal microbiota did not change during the estrous cycle. Core equine vaginal microbiome consisted of Firmicutes, Bacteroidetes, Proteobacteria and Actinobacteria at the phylum level. At the genus level it was defined by Porphyromonas, Campylobacter, Arcanobacterium, Corynebacterium, Streptococcus, Fusobacterium, uncultured Kiritimatiaellae and Akkermansia. Lactobacillus comprised only 0.18% of the taxonomic composition in estrus and 0.37% in diestrus. No differences in the relative abundance of the most abundant phylum or genera were observed between estrus and diestrus samples.
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影响因子：
3.5
作者：
Abraham A;Pedregosa F;Eickenberg M;Gervais P;Mueller A;Kossaifi J;Gramfort A;Thirion B;Varoquaux G
通讯作者：
Varoquaux G
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
5.2
作者：
Clemmons BA;Reese ST;Dantas FG;Franco GA;Smith TPL;Adeyosoye OI;Pohler KG;Myer PR
通讯作者：
Myer PR
影响因子：
2.8
作者：
Groppetti, D.;Pecile, A.;Martino, P. A.
通讯作者：
Martino, P. A.
影响因子：
1.7
作者：
Manes, J.;Campero, C.;Ungerfeld, R.
通讯作者：
Ungerfeld, R.