The smooth muscle cell. II. Growth of smooth muscle in culture and formation of elastic fibers.
The smooth muscle cell. II. Growth of smooth muscle in culture and formation of elastic fibers.
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DOI：
10.1083/jcb.50.1.172
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发表时间：
1971-07
期刊：
影响因子：
--
通讯作者：
Ross R
中科院分区：
文献类型：
--
作者：
Ross R
取未成熟的豚鼠主动脉内中膜和内膜，在细胞培养中培养8wk。在培养过程中，细胞在生长的各个阶段都保持着平滑肌的形态。在生长到融合后，它们在多个重叠的层中生长。培养4wk时，细胞层间隙内出现微纤维(110A)。细胞周围也可见基底膜样物。对微原纤维的分析表明，它们的氨基酸组成与完整弹性纤维的微原纤维蛋白相似。结合放射自显影观察主动脉平滑肌合成和分泌细胞外蛋白的能力，证明该细胞是结缔组织合成细胞。
Smooth muscle derived from the inner media and intima of immature guinea pig aorta were grown for up to 8 wk in cell culture. The cells maintained the morphology of smooth muscle at all phases of their growth in culture. After growing to confluency, they grew in multiple overlapping layers. By 4 wk in culture, microfibrils (110 A) appeared within the spaces between the layers of cells. Basement membrane-like material also appeared adjacent to the cells. Analysis of the microfibrils showed that they have an amino acid composition similar to that of the microfibrillar protein of the intact elastic fiber. These investigations coupled with the radioautographic observations of the ability of aortic smooth muscle to synthesize and secrete extracellular proteins demonstrate that this cell is a connective tissue synthetic cell.
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DOI：
10.1016/s0368-1319(65)80006-0
发表时间：
1965-01-01
期刊：
JOURNAL OF ATHEROSCLEROSIS RESEARCH
影响因子：
--
作者：
MYASNIKO.AL;BLOCK, YE
通讯作者：
BLOCK, YE
影响因子：
3.1
作者：
POLLAK, OJ;KASAI, T
通讯作者：
KASAI, T
影响因子：
--
作者：
BO, WJ;ODOR, DL;ROTHROCK, M
通讯作者：
ROTHROCK, M
DOI：
10.1016/s0368-1319(61)80034-3
发表时间：
1961-01-01
期刊：
JOURNAL OF ATHEROSCLEROSIS RESEARCH
影响因子：
--
作者：
KOKUBU, T;POLLAK, OJ
通讯作者：
POLLAK, OJ
DOI：
10.1083/jcb.50.1.159
发表时间：
1971-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ross R;Klebanoff SJ
通讯作者：
Klebanoff SJ