Phenolic-Enriched Collagen Fibrillar Coatings on Titanium Alloy to Promote Osteogenic Differentiation and Reduce Inflammation.
Phenolic-Enriched Collagen Fibrillar Coatings on Titanium Alloy to Promote Osteogenic Differentiation and Reduce Inflammation.
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DOI：
10.3390/ijms21176406
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发表时间：
2020-09-03
影响因子：
5.6
通讯作者：
Gurzawska-Comis KA
中科院分区：
文献类型：
--
作者：
Mieszkowska A;Beaumont H;Martocq L;Koptyug A;Surmeneva MA;Surmenev RA;Naderi J;Douglas TEL;Gurzawska-Comis KA
生物材料表面上生物分子的吸附可以促进它们与周围组织的整合，而不改变它们的体积特性。对于骨重建中的生物材料来说，促进成骨分化和减少炎症是理想的。原纤维涂层很有趣，因为原纤维具有高表面积体积比，有助于吸附和粘附。原纤维还可以作为固定具有生物活性的生物分子的基质，例如酚类化合物间苯三酚（PG），海洋多酚的亚基。这项工作的目的是研究 PG 涂层对成纤维细胞和成骨细胞样细胞的影响，以增加钛种植体的骨整合。在增材制造 (AM) 生成的钛合金 (Ti6Al4V) 支架上生产含有低浓度和高浓度 PG 的胶原原纤维涂层。这些涂层，尤其是富含 PG 的涂层，可降低疏水性并调节人骨肉瘤 SaOS-2 和小鼠胚胎成纤维细胞 3T3 细胞系的行为。成骨细胞和成纤维细胞在富含 PG 的涂层上都能很好地扩散和粘附。涂层显着降低了炎症反应。此外，高PG浓度的胶原蛋白涂层促进成骨分化。因此，用 PG 富集胶原纤维涂层是改善骨科和牙科中 Ti6Al4V 植入物骨接触的一种有前景的策略，值得进一步研究。
The adsorption of biomolecules on biomaterial surfaces can promote their integration with surrounding tissue without changing their bulk properties. For biomaterials in bone reconstruction, the promotion of osteogenic differentiation and reduction of inflammation are desirable. Fibrillar coatings are interesting because of fibrils’ high surface area-volume ratio, aiding adsorption and adhesion. Fibrils also serve as a matrix for the immobilization of biomolecules with biological activity, such as the phenolic compound phloroglucinol (PG), the subunit of marine polyphenols. The aim of this work was to investigate the influence of PG coatings on fibroblast- and osteoblast-like cells to increase the osseointegration of titanium implants. Collagen fibril coatings, containing PG at low and high concentrations, were produced on titanium alloy (Ti6Al4V) scaffolds generated by additive manufacturing (AM). These coatings, especially PG-enriched coatings, reduced hydrophobicity and modulated the behavior of human osteosarcoma SaOS-2 and mouse embryonic fibroblast 3T3 cell lines. Both osteoblastic and fibroblastic cells spread and adhered well on PG-enriched coatings. Coatings significantly reduced the inflammatory response. Moreover, osteogenic differentiation was promoted by collagen coatings with a high PG concentration. Thus, the enrichment of collagen fibril coatings with PG is a promising strategy to improve Ti6Al4V implants for bone contact in orthopedics and dentistry and is worthy of further investigation.
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影响因子：
3.7
作者：
Lopes G;Sousa C;Silva LR;Pinto E;Andrade PB;Bernardo J;Mouga T;Valentão P
通讯作者：
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影响因子：
11.2
作者：
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通讯作者：
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影响因子：
4.1
作者：
Fraser, JHE;Helfrich, MH;Ralston, SH
通讯作者：
Ralston, SH
影响因子：
4.3
作者：
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通讯作者：
Kim, Se-Kwon
影响因子：
3.4
作者：
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通讯作者：
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