Disruption of olfactory receptor neuron patterning in Scutoid mutant Drosophila.
Disruption of olfactory receptor neuron patterning in Scutoid mutant Drosophila.
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DOI：
10.1016/j.mcn.2010.09.008
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发表时间：
2011-01
影响因子：
3.5
通讯作者：
Ray, A.
中科院分区：
文献类型：
--
作者：
Tom, W.;de Bruyne, M.;Haehnel, M.;Carlson, J. R.;Ray, A.
嗅觉神经元表现出极其多样化的细胞类型，每个细胞通常表达果蝇 60 个气味受体 (Or) 基因大家族中的一个成员。人们对产生这种细胞多样性定型模式的发育过程和转录因子知之甚少。在这里，我们研究了一种不寻常的显性突变体 Scutoid 嗅觉系统功能缺陷的分子和细胞基础。我们表明，这些缺陷映射到支配天线上特定形态类别感器的嗅觉神经元，即大型基本学。分子分析表明，表达特定类别受体基因的神经元存在缺陷，这些基因映射到大型基本感器。先前的研究表明，在 Scutoid 突变体中，转录抑制因子 snail 的编码区易位在无眼启动子附近，导致 snail 在发育中的眼触角盘中错误表达。我们发现，发育中的嗅觉神经元中 snail 的异位表达会导致触角大基本神经元的严重缺陷，这支持了 Scutoid 中的主要嗅觉表型是由 snail 的错误表达引起的模型。
Olfactory neurons show an extreme diversity of cell types with each cell usually expressing one member from a large family of 60 Odorant receptor (Or)genes in Drosophila. Little is known about the developmental processes and transcription factors that generate this stereotyped pattern of cellular diversity. Here we investigate the molecular and cellular basis of defects in olfactory system function in an unusual dominant mutant, Scutoid. We show that the defects map to olfactory neurons innervating a specific morphological class of sensilla on the antenna, large basiconics. Molecular analysis indicates defects in neurons expressing specific classes of receptor genes that map to large basiconic sensilla. Previous studies have shown that in Scutoid mutants the coding region of the transcriptional repressor snail is translocated near the no-ocelli promoter, leading to misexpression of snail in the developing eye-antenna disc. We show that ectopic expression of snail in developing olfactory neurons leads to severe defects in neurons of the antennal large basiconics supporting the model that the dominant olfactory phenotype in Scutoid is caused by misexpression of snail.
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