Unveil early-stage nanocytotoxicity by a label-free single cell pH nanoprobe.
Unveil early-stage nanocytotoxicity by a label-free single cell pH nanoprobe.
复制标题
通过无标记的单细胞pH纳米探针揭示早期纳米毒性的早期纳米细胞毒性。
DOI：
10.1039/d0an01437k
复制
发表时间：
2020-11-09
期刊：
影响因子：
--
通讯作者：
Ma Y
中科院分区：
文献类型：
--
作者：
Yang Q ;Cristea A ;Roberts C ;Liu K ;Song Y ;Xiao H ;Shi H ;Ma Y
单细胞分析是一个新兴的研究领域，旨在通过克服细胞间的异质性来揭示微妙的细胞状态和潜在的机制。然而，目前的单细胞研究方法高度依赖于细胞破坏性协议，不能顺序显示细胞事件的进展。我们实验室最近开发的pH纳米探针在概念上显示了其在没有细胞标记或破坏的情况下检测细胞内pH（pHi）的能力。在本研究中，我们将纳米颗粒（NPs）的细胞毒性作为细胞异质性的典型例子，以证明pH纳米探针在解释细胞状态方面的实用性。采用三种类型的纳米颗粒（CeO 2，TiO 2和SiO2）产生不同的毒性作用。结果表明，传统的检测方法--包括细胞活力、细胞内ROS生成和线粒体内膜去极化--不仅未能准确及时地报告纳米毒性，而且得出了令人困惑或误导的结论。pH纳米探针揭示了由NPs诱导的明确的pHi变化，这与通过透射电子显微镜（TEM）成像发现的细胞损伤很好地对应。此外，我们的研究结果揭示了在NP-细胞相互作用的早期阶段，SiO2 NPs对细胞的意外破坏性影响。所开发的新型pH纳米探针表现出以最小侵入性在单细胞分辨率下的快速感测能力。因此，它可能成为单细胞研究和精准医学等领域广泛应用的一种有前途的替代方案。开发的pH纳米探针揭示了以前未知的纳米材料特性（例如，破坏性的细胞毒性）。
Single-cell analysis is an emerging research area that aims to reveal delicate cellular status and underlying mechanisms by conquering the intercellular heterogeneity. Current single-cell research methods, however, are highly dependent on cell-destructive protocols and cannot sequentially display the progress of cellular events. A recently developed pH nanoprobe in our lab conceptually showed its ability to detect intracellular pH (pHi) without cell labeling or disruption. In the present study, we took the cytotoxicity of nanoparticles (NPs) as a typical example of cell heterogeneity, to testify the practicality of the pH nanoprobe in interpreting cell status. Three types of NPs (CeO2, TiO2, and SiO2) were employed to generate varied toxic effects. Results showed that the traditional assays - including cell viability, intracellular ROS generation, and mitochondrial inner membrane depolarization - not only failed to report the nanotoxicity accurately and timely, but also drew confusing or misleading conclusions. The pH nanoprobe revealed explicit pHi changes induced by the NPs, which corresponded well with the cell damages found by the transmission electron microscopic (TEM) imaging. Besides, our results unveiled an unexpectedly devastating effect of SiO2 NPs on cells during the early stage NP-cell interaction. The developed novel pH nanoprobe demonstrated a rapid sensing capability at single-cell resolution with minimum invasiveness. Therefore, it may become a promising alternative for a wide range of applications in areas such as single-cell research and precision medicine. The developed pH nanoprobe unveiled nanomaterial properties that previously unknown (e.g., devastating cytotoxicity) via real-time label-free monitoring on single cells.
登录
查看更多内容
影响因子：
4.3
作者：
Dan, Yongbo;Ma, Xingmao;Shi, Honglan
通讯作者：
Shi, Honglan
影响因子：
5
作者：
Alaraby, Mohamed;Hernandez, Alba;Marcos, Ricard
通讯作者：
Marcos, Ricard
影响因子：
48
作者：
Angermueller C;Clark SJ;Lee HJ;Macaulay IC;Teng MJ;Hu TX;Krueger F;Smallwood S;Ponting CP;Voet T;Kelsey G;Stegle O;Reik W
通讯作者：
Reik W
基于金纳米簇的荧光生物传感器，用于癌细胞靶向成像和细胞内 pH 值的比例测定
影响因子：
12.6
作者：
Ding, Changqin;Tian, Yang
通讯作者：
Tian, Yang
影响因子：
4.5
作者：
BUCKLER, KJ;VAUGHANJONES, RD
通讯作者：
VAUGHANJONES, RD