Cell-based approaches to the engineering of vascularized bone tissue.
Cell-based approaches to the engineering of vascularized bone tissue.
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DOI：
10.1016/j.jcyt.2013.06.005
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发表时间：
2013-11
期刊：
影响因子：
4.5
通讯作者：
Stegemann JP
中科院分区：
文献类型：
--
作者：
Rao RR;Stegemann JP
本文综述了最近的努力，创造血管化骨组织在体外和体内使用细胞为基础的治疗方法。大面积骨折的治疗是一个严重的临床问题，在美国，大约10%的骨折并发延迟愈合或骨不连。使用生长因子和基因递送的治疗方法已经显示出一些前景，但结果是可变的，并且出现了临床并发症。基于细胞的治疗提供了概括骨愈合级联的关键组成部分的潜力，其涉及血管和新骨组织的伴随再生。出于这个原因，成骨细胞和血管生成细胞类型已经在共培养物中组合，以利用每种细胞类型的功能，并促进异型相互作用。在2D和3D系统中的实验已经提供了对成骨细胞和血管生成细胞相互作用以形成血管化骨的机制的深入了解，并且这些方法已经被转化为小动物研究中的异位和原位模型。这些研究产生的知识将为下一代临床前研究提供信息和促进，这是将基于细胞的骨科修复策略推向临床所需的。细胞治疗在修复大面积缺血性骨缺损中的应用正在迅速发展，有望为这些具有挑战性的临床问题提供新的治疗方法。
This review summarizes recent efforts to create vascularized bone tissue in vitro and in vivo using cell-based therapy approaches. The treatment of large and recalcitrant bone wounds is a serious clinical problem, and in the United States approximately 10% of all fractures are complicated by delayed or non-union. Treatment approaches using growth factor and gene delivery have shown some promise, but results are variable and clinical complications have arisen. Cell-based therapies offer the potential to recapitulate key components of the bone healing cascade, which involves concomitant regeneration of vasculature and new bone tissue. For this reason, osteogenic and vasculogenic cell types have been combined in co-cultures to capitalize on the function of each cell type, and to promote heterotypic interactions. Experiments in both 2D and 3D systems have provided insight into the mechanisms by which osteogenic and vasculogenic cells interact to form vascularized bone, and these approaches have been translated to ectopic and orthotopic models in small animal studies. The knowledge generated by these studies will inform and facilitate the next generation of pre-clinical studies, which are needed to move cell-based orthopaedic repair strategies into the clinic. The science and application of cytotherapy for repair of large and ischemic bone defects is developing rapidly, and promises to provide new treatment methods for these challenging clinical problems.
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影响因子：
4.1
作者：
Buschmann, Johanna;Welti, Manfred;Calcagni, Maurizio
通讯作者：
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影响因子：
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