High frequency plant regeneration from microspore-derived embryos of ornamental kale (Brassica oleracea L. var. acephala)
High frequency plant regeneration from microspore-derived embryos of ornamental kale (Brassica oleracea L. var. acephala)
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观赏羽衣甘蓝（Brassica oleracea L. var. acephala）小孢子衍生胚胎的高频植物再生
DOI：
10.1016/j.scienta.2011.06.029
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发表时间：
2011-08
影响因子：
4.3
通讯作者：
Feng, Hui
中科院分区：
文献类型：
--
作者：
Wang, Yushu;Tong, Yan;Li, Yuefei;Zhang, Yun;Zhang, Jun;Feng, Jianyun;Feng, Hui
The aim of this work was to study the effect of solid medium, developmental stage, embryonic age, cold treatment and additives to the medium on plant regeneration from microspore-derived embryos in four F1hybrids of ornamental kale (Brassica oleracea L. var. acephala). The results showed that all of the cultivars responded best when the embryos were cultured in solidified B5 medium with 1% agar. Optimal regeneration was gained when cotyledonary embryos were cultured for 25 days. Cold treatment significantly improved plant regeneration with a frequency of up to 79.0% under 4°C for 2d or 5d. The addition of 3.0 or 5.0mg/L silver nitrate (AgNO3) increased the frequency of plant regeneration. In the Zhouyehongxin cultivar, the frequency of plantlet development reached 84.4%. The addition of activated charcoal reduced embryo hyperhydricity.
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DOI：
10.1007/978-94-017-1293-4_30
发表时间：
2003
期刊：
--
影响因子：
--
作者：
C. J.;Silva Dias
通讯作者：
C. J.;Silva Dias
影响因子：
1.5
作者：
G. Zhang;D. Zhang;G. Tang;Y. He;W. Zhou
通讯作者：
G. Zhang;D. Zhang;G. Tang;Y. He;W. Zhou
影响因子：
1.9
作者：
J. Dias
通讯作者：
J. Dias
DOI：
--
发表时间：
2006
期刊：
--
影响因子：
--
作者：
Zhu Hong-yi;C. Wei-hong
通讯作者：
Zhu Hong-yi;C. Wei-hong
DOI：
10.1007/bf02632058
发表时间：
2007
期刊：
In Vitro Cellular & Developmental Biology - Plant
影响因子：
--
作者：
Bin Huang;S. Bird;R. Kemble;B. Miki;W. Keller
通讯作者：
Bin Huang;S. Bird;R. Kemble;B. Miki;W. Keller