Genomic data mining reveals a rich repertoire of transport proteins in Streptomyces.
Genomic data mining reveals a rich repertoire of transport proteins in Streptomyces.
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基因组数据挖掘揭示了链霉菌中丰富的转运蛋白
DOI：
10.1186/s12864-016-2899-4
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发表时间：
2016-08-22
期刊：
影响因子：
4.4
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Zhou Z;Sun N;Wu S;Li YQ;Wang Y
背景：链霉菌是居住在土壤中的革兰氏阳性细菌，是制药工业中使用的抗生素的主要生产者。链霉菌中异常强大的转运系统的进化使它们能够适应复杂的土壤环境。结果：我们的比较基因组分析显示，11种链霉菌中每一种都拥有761-1258种转运蛋白的丰富库，占每个蛋白质组的10.2%至13.7%。这些转运蛋白可分为7个功能类和171个转运蛋白家族。其中，atp结合盒（ABC）超家族和主要促进者超家族（MFS）占链霉菌转运蛋白总数的40%以上。它们在营养摄取和底物分泌，特别是药物和毒物的外排中都起着重要作用。11种链霉菌的进化灵活性体现在两种主要蛋白质易位途径中转运蛋白的谱系特异性分布上：总分泌（Sec）途径和双精氨酸易位（Tat）途径。结论：我们的研究结果提供了11种链霉菌的运输系统目录。这些广泛的运输系统是复杂过程的重要介质，包括营养摄取，元素浓度平衡，药物和毒素的外排以及蛋白质的及时有序分泌。链霉菌广泛应用于抗生素生产和重组蛋白的异源表达，对其运输系统的更好理解将有助于优化其制药和工业应用的生产工艺。
Background:Streptomycetes are soil-dwelling Gram-positive bacteria that are best known as the major producers of antibiotics used in the pharmaceutical industry. The evolution of exceptionally powerful transporter systems in streptomycetes has enabled their adaptation to the complex soil environment.Results:Our comparative genomic analyses revealed that each of the eleven Streptomyces species examined possesses a rich repertoire of from 761-1258 transport proteins, accounting for 10.2 to 13.7 % of each respective proteome. These transporters can be divided into seven functional classes and 171 transporter families. Among them, the ATP-binding Cassette (ABC) superfamily and the Major Facilitator Superfamily (MFS) represent more than 40 % of all the transport proteins in Streptomyces. They play important roles in both nutrient uptake and substrate secretion, especially in the efflux of drugs and toxicants. The evolutionary flexibility across eleven Streptomyces species is seen in the lineage-specific distribution of transport proteins in two major protein translocation pathways: the general secretory (Sec) pathway and the twin-arginine translocation (Tat) pathway.Conclusions:Our results present a catalog of transport systems in eleven Streptomyces species. These expansive transport systems are important mediators of the complex processes including nutrient uptake, concentration balance of elements, efflux of drugs and toxins, and the timely and orderly secretion of proteins. A better understanding of transport systems will allow enhanced optimization of production processes for both pharmaceutical and industrial applications of Streptomyces, which are widely used in antibiotic production and heterologous expression of recombinant proteins.
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影响因子：
14.9
作者：
Ren Q;Chen K;Paulsen IT
通讯作者：
Paulsen IT
影响因子：
3.2
作者：
Ohnishi, Yasuo;Ishikawa, Jun;Horinouchi, Sueharu
通讯作者：
Horinouchi, Sueharu
影响因子：
4.2
作者：
Getsin I;Nalbandian GH;Yee DC;Vastermark A;Paparoditis PC;Reddy VS;Saier MH Jr
通讯作者：
Saier MH Jr
运输蛋白演变的途径：最近的进步。
影响因子：
3.7
作者：
Lam VH;Lee JH;Silverio A;Chan H;Gomolplitinant KM;Povolotsky TL;Orlova E;Sun EI;Welliver CH;Saier MH Jr
通讯作者：
Saier MH Jr
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.