A naturally occurring defective DNA satellite associated with a monopartite begomovirus: evidence for recombination between alphasatellite and betasatellite.
A naturally occurring defective DNA satellite associated with a monopartite begomovirus: evidence for recombination between alphasatellite and betasatellite.
复制标题
与单部分 Begomovirus 相关的天然存在的缺陷 DNA 卫星：α 卫星和 Beta 卫星之间重组的证据
DOI：
10.3390/v5092116
复制
发表时间：
2013-09-06
期刊：
影响因子：
--
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Huang C;Xie Y;Zhao L;Ren H;Li Z
单一性begomovirus及其相关卫星病毒形成了独特的疾病复合体，对全世界的农业构成严重威胁。众所周知，频繁的重组有助于双病毒的多样化和进化。在本研究中，我们在一株自然感染的烟草植株上发现了一个与烟草卷叶云南病毒（TbLCYNV）相关的新型缺陷卫星分子（RecSat）。序列分析表明，Recsat全长754个核苷酸，是一个包含α -卫星和β -卫星序列的嵌合体，包含β -卫星保守区和α -卫星茎环结构。重组分析表明，RecSat是由三个独立的重组事件引起的，可能涉及番茄黄叶卷曲中国betassatellite， Ageratum黄脉中国betassatellite和烟草卷曲芽alphasatsatellite。RecSat与TbLCYNV共接种在烟叶中诱导的症状与TbLCYNV单独诱导的症状没有区别。Southern blot杂交结果表明，TbLCYNV可以稳定地在benthamiana植物中进行交叉复制，并抑制了辅助病毒和共接种的α -卫星的积累。我们的研究结果首次提供了双病毒复合体中两种不同类型卫星之间重组的证据，并强调了重组是双病毒进化的驱动力。
Monopartite begomoviruses and their associated satellites form unique disease complexes that have emerged as a serious threat to agriculture worldwide. It is well known that frequent recombination contributes to the diversification and evolution of geminiviruses. In this study, we identified a novel defective satellite molecule (RecSat) in association with Tobacco leaf curl Yunnan virus (TbLCYNV) in a naturally infected tobacco plant. Sequence analysis showed that Recsat comprises 754 nucleotides in size and is a chimera involving alphasatellite and betasatellite sequences, containing both betasatellite-conserved region and alphasatellite stem-loop structure. Recombination analysis revealed that RecSat has arisen from three independent recombination events likely involving Tomato yellow leaf curl China betasatellite, Ageratum yellow vein China betasatellite and Tobacco curly shoot alphasatellite. Co-inoculation of RecSat with TbLCYNV induced symptoms indistinguishable from those induced by TbLCYNV alone in Nicotiana benthamiana. Southern blot hybridization showed that RecSat could be trans-replicated stably in N. benthamiana plants by TbLCYNV, and impaired the accumulation of helper virus and co-inoculated alphasatellite. Our results provide the first evidence for recombination between two distinct types of satellites among geminivirus complex and highlight recombination as a driving force for geminivirus evolution.
登录
查看更多内容
影响因子：
5.4
作者：
Cui, XF;Tao, XR;Zhou, XP
通讯作者：
Zhou, XP
影响因子：
3.5
作者：
Eini, Omid;Dogra, Satish;Rezaian, M. Ali
通讯作者：
Rezaian, M. Ali
影响因子：
3.7
作者：
Paprotka, T.;Metzler, V.;Jeske, H.
通讯作者：
Jeske, H.
影响因子：
1.5
作者：
Cui, XF;Xie, Y;Zhou, XP
通讯作者：
Zhou, XP
影响因子：
--
作者：
Xie, Y;Zhou, XP;Li, GX
通讯作者：
Li, GX