The association between fecal microbiota, age and endoparasitism in adult alpacas.
The association between fecal microbiota, age and endoparasitism in adult alpacas.
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DOI：
10.1371/journal.pone.0272556
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
内寄生是羊驼发病和死亡的主要原因，随着驱虫药耐药性的不断出现。该研究的目的是将线虫负担和选定的宿主表型特征（如年龄和体重）与成年羊驼肠道微生物群的组成相关联。在3个不同的时间点（8.8 mg/kg盐酸左旋咪唑口服给药前和给药后，以及4.6个月后），在一个农场从102头健康成年（2.1-11.2岁）羊驼经直肠采集粪便样本。使用16 S扩增子测序表征粪便细菌微生物群的概况。使用相关样本分析比较系列临床检查和粪便虫卵计数。相同管理的健康羊驼的粪便微生物群具有高度的时间稳定性，因为α和β多样性在采样时间点之间显著相关。每个时间点采集的样本之间的成对β多样性较低，范围为0.16-0.21 UniFrac距离单位。在左旋咪唑处理后14天收集的样品中，圆线虫感染（包括血矛线虫属、奥斯特线虫属、毛圆线虫属）的强度仅与微生物群组成显著相关。相似性分析显示，来自驱虫剂应答者或非应答者的微生物群没有聚类。羊驼年龄解释了粪便微生物群变异的最大比例，并且是粪便微生物群分类组成的唯一一贯显著的预测因子，通过影响相对拟杆菌门和厚壁菌门丰度的比率。厚壁菌门（Firmicutes），主要是梭菌目（Clostridiales），是所有标本中最丰富的类群。
Endoparasitism is a major cause of morbidity and mortality in alpacas (Lama pacos), with growing emergence of anthelmintic resistance. The purpose of the study was to correlate nematode worm burden and selected host phenotypic characteristics, such as age and weight, with the composition of the intestinal microbiota of adult alpacas. Fecal samples were collected per rectum from 102 healthy adult (2.1–11.2 years) alpacas at 3 separate timepoints (pre- and post-treatment with 8.8 mg/kg oral Levamisole HCL, and 4.6 months later) at a single farm. The profile of the fecal bacterial microbiota was characterized using 16S amplicon sequencing. Serial clinical exams and fecal egg counts were compared using related-samples analyses. The fecal microbiota of identically managed, healthy alpacas was characterized by a high level of temporal stability, as both α and β-diversity significantly correlated between sampling timepoints. Pairwise β-diversity between samples collected at each timepoint was low, ranging from 0.16–0.21 UniFrac distance units. The intensity of strongylid nematode infection (including Haemonchus, Ostertagia, Trichostrongylus) was only significantly correlated with microbiota composition in samples collected 14 days after treatment with levamisole. Analysis of similarity revealed no clustering of microbiota from anthelmintic responders or non-responders. Alpaca age explained the largest proportion of fecal microbiota variation and was the only consistently significant predictor of fecal microbiota taxonomic composition, by impacting the ratio of relative Bacteroidetes and Firmicutes abundance. Firmicutes, mostly Clostridiales, was the most abundant taxon across all collections.
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影响因子：
5.2
作者：
Leung JM;Graham AL;Knowles SCL
通讯作者：
Knowles SCL
影响因子：
3.7
作者：
Dougal K;de la Fuente G;Harris PA;Girdwood SE;Pinloche E;Geor RJ;Nielsen BD;Schott HC 2nd;Elzinga S;Newbold CJ
通讯作者：
Newbold CJ
影响因子：
2.7
作者：
Chao, Ruimin;Xia, Chengqiang;Ren, Youshe
通讯作者：
Ren, Youshe
影响因子：
4
作者：
Kaplan, R. M.;Vidyashankar, A. N.;Terrill, T. H.
通讯作者：
Terrill, T. H.
影响因子：
3.2
作者：
Rashid, Mohammed H.;Vaughan, Jane L.;Jabbar, Abdul
通讯作者：
Jabbar, Abdul